木薯花粉活力测定及验证试验

Determining and testing of the cassava pollen vigor

以6个木薯品种为供试材料,分别采用碘-碘化钾(I2-KI)染色法、氯化三苯四氮唑(TTC)染色法、醋酸洋红染色法、离体萌芽法对其花粉活力进行测定,以自然授粉法和人工杂交法对其进行验证。结果显示:I2-KI染色法、醋酸洋红染色法、氯化三苯四氮唑(TTC)染色法、离体萌芽法测定花粉平均活力分别是96.8%、96.4%、1.8%、0～1.12%;人工杂交授粉率为0%～0.34%,自然授粉率则为38.2%～62.9%。验证试验表明:供试木薯花粉具有活力,CCT法和离体萌芽法,不能正确反映花粉活力状况,碘-碘化钾和醋酸洋红染色法能测出花粉活力,但能否则正确反映花粉活力,有待进一步验证。

This paper determined pollen vigor of six cassava varieties by I2-KI,TTC,aceto-carmine staining method and tissue culture,then tested them by natural pollination and artificial pollination. The results showed that the pollen vigor was 96.8%,96.4%, 1.8% and 0-1.12% respectively by TTC,tissue culture,aceto-earmine staining and I2-KI method. The rate of ar：ifieial hybridized pollination was 0%-0.34%. The rate of natural pollination was 38.2%-62.9%. The pollen of these cassave had vigor.The method of CCT and tissue culture could not rightly reflect the situation of pollen vigor of eassave.The staining method of aeeto-earmine and I2-KI could test pollen vigor, and need to be further proved.