皱纹盘鲍脏器糖蛋白结构的初步鉴定及其免疫活性研究

Preliminary configuration identification and immuno-enhancing activity of Haliotis discus hannai Ino visceral glycoprotein

目的分析鉴定皱纹盘鲍脏器糖蛋白AGP-Ⅲ初级结构并探讨脏器糖蛋白粗品AGP的免疫活性。方法采用高效液相色谱法、氨基酸组成分析法、红外光谱法、β-消除反应对AGP-Ⅲ进行初步分析;建立小鼠免疫低下模型,探讨AGP不同剂量对小鼠免疫功能的调节作用。结果AGP-Ⅲ的单糖组成有岩藻糖、半乳糖、氨基葡萄、甘露糖、氨基半乳糖、木糖、葡萄糖和鼠李糖。AGP-Ⅲ含有17种氨基酸,缬氨酸含量最高,其次为谷氨酸、甘氨酸。AGP-Ⅲ中存在糖蛋白的特征吸收峰及β折叠结构,糖肽键类型为O-糖肽键,糖苷键类型为吡喃型。AGP中、高剂量组能显著提高免疫低下小鼠的细胞免疫和非特异性免疫功能。结论AGP-Ⅲ有糖蛋白的特征结构,AGP在适宜剂量条件下可提高小鼠的免疫能力。

Objective The configuration analysis and immuno-enhancing activity of purified Haliotis discus hannai Ino visceral glycoprotein AGP-Ⅲ were investigated in this study.Methods Methods of HPLC,amino acids analysis,IR analysis,β-elimination reaction were used.Results Monosaccharide compositions of AGP-Ⅲ were Man,GlcNac,Rha,GalNAc,Glc,Gal,Xyl and Fuc.Seventeen kinds of amino acids were determined in AGP-Ⅲ,and it was rich in Val,Glu and Gly.AGP-Ⅲ shared with a typical absorption property of glycoprotein in a pyranose β-glycosidic and O-glycoside linkage.The medium dosage（50 mg·kg^-1） and high dosage（100 mg·kg^-1） of AGP could promote immuological function both in celluar and non-specific immunity in immunodepression mice.Conclusion AGP-Ⅲ had typical configuration of glycoprotein and the Haliotis discus hannai Ino visceral glycoprotein could enhance the immunity of mice.