几种小麦雄性不育系及其保持系、恢复系的酯酶同工酶比较

A Comparative Study of Esterase Isozyme in Flag Leaves and Young Spikelets among Some Common Wheat CMS Lines and Their Maintainers and Restorers

为了阐明同核不同细胞质的小麦雄性不育系、保持系、恢复系之间酯酶同工酶的异同,通过聚丙烯酰胺凝胶电泳(PAGE)对T型、Q型、AL型三种小麦雄性不育系及其保持系、恢复系等合计35个材料的孕穗期旗叶和幼小穗中的酯酶同工酶进行了研究。结果表明,保持系与恢复系在旗叶和幼小穗的酯酶同工酶上未发现明显差异。但不同不育系(TA92-8、QA92-8、AL92-8)之间以及与其同核保持系(绵阳92-8)之间存在差异。其中,Q型不育系在旗叶和幼小穗酯酶同工酶上与同核保持系一致,但T型、AL型不育系比其保持系多2条旗叶期酯酶同工酶谱带(ESTa-8,9)。上述结果说明,不育系与保持系之间的谱带差异可能与特定核质互作有关。本研究为T型、Q型、AL型不育系的区分提供了一个有效的方法。

In order to study the differences of esterase spectrum among different cytoplasmic male ster- ile lines with the same nuclear and among A-lines, B-lines and R-lines, the easterase isozymes （EST） in flag leaf and young spikelet of T-, Q-, AL-type male-sterile lines with same nuclear and their B- lines, and R-lines etc totally 35 common wheat lines were studied by means of PAGE analysis. The results indicated young spike type sterile yang 92-8）. ets that there are between B-lines ines and between s Among them, Q-t young spikelets to its Ta-8,9） in flag leaves malnta more no obviously differences of the esterase isozyme in the flag leaf and and R-lines. There are, however, some differences among T, Q, AL- terile lines （QA92-8, TA92-8, and AL92-8） and their B-line （Mian- ype sterile line has similar EST isozyme spectrum in flag leaves and iner, and T-type and AL-type sterile lines have two additive bands （ES- than their maintainer, indicating special nuclear-cytoplasm interaction of the different sterile lines and an identification method for different cytoplasm sterile lines.