摘要
[目的]分别以甘薯品种徐薯22的叶片与茎尖作为外植体,研究利用不同外植体通过体细胞胚胎再生途径得到再生植株的方法。[方法]将徐薯22的叶片和茎尖分别置于MSB和MSD培养基中诱导胚性愈伤,再将胚性愈伤置于MS培养基培养,观察体细胞胚的发生情况,最后对不同外植体得到的植株再生频率进行比较。[结果]用叶片作为外植体得到的胚性愈伤平均诱导频率为95.69%,而茎尖的则为30.56%;不同外植体在体细胞胚发生途径中的形态特征有一定差异;用叶片作为外植体的植株再生频率为60.61%,用茎尖的则为22.00%,且采用不同外植体诱导得到的再生植株无形态变化。[结论]在该试验中,体细胞胚的发生及植株再生的最适外植体为甘薯品种徐薯22的叶片。
[Objective] This study aimed to regenerate plants of sweet potato (Ipomoea batatas) cultivar Xushu22 via somatic embryogenesis, using leaf and shoot apex as explants. [Method] The leaf and shoot apex of Xushu 22 were separately cultured on MSB medium and MSD medium. The induced embryogenic calluses were then cultured on MS medium. The regeneration frequency of leaf and shoot apex explants were respectively calculated. [Result] The average frequency of leaf explants developing somatic callus was 95.69% compared to 30.56% in case of shoot apex explants. There were different types of morphogenic structures in the process of somatic embryo development. Leaf explants gave a high regeneration frequency to 60.61%, while the regeneration frequency of shoot apices was 22%. In addition, no morphological variations were observed in the regeneration plants. [Conclusion] Leaf explant was better than shoot apices in plant regeneration of Xushu22 via somatic embryogenesis.
基金
国家863计划项目(2011AA100607)
重庆市科技攻关重点项目(2009AB1185)~~
