摘要
[目的]对卷柏的化学成分进行分离与鉴定。[方法]从传统中草药卷柏全草中分离得到化合物,采用柱层析色谱法及制备型薄层色谱法对化合物进行纯化,再通过核磁及质谱分析的方法确定化合物的结构,最后用MTT法测试化合物的抗癌活性。[结果]试验分离得到了7个化合物,分别鉴定为巴西棕榈酸(1)、葡萄糖(2)、β-谷甾醇(3)、穗花杉双黄酮(4)、selaginellin(5)、selaginellin M(6)和selaginellin A(7);并且在所测试的3种化合物中,以化合物7抑制HeLa癌细胞生长的活性最好。[结论]为更好地开发利用卷柏植物资源提供了依据。
[Objective] This study aimed to isolate and identify the chemical constituents of Selaginella tamariscina. [Method] The chemical compounds isolated from the whole plant of traditional Chinese herb S. tamariscina were purified through column chromatography and preparative thin-layer chromatography, and then chemical structures of the compounds were determined by NMR and mass spectrometry. Finally, the anticancer activity of the compounds 5, 6 and 7 was measured by MTT assay. [Result] Seven compounds, containing carnaubic acid (1), glucose (2), β-sitosterol (3), amentoflavone (4), selaginellin (5), selaginellin M (6) and selaginellin A (7), were isolated from methanol extracts of S. tamariscina. Among the three tested compounds, compound 7 exhibited the most effective activity to inhibit the HeLa cell growth. [Conclusion] This study will provide a basis for better development and utilization of Selaginella plant resources.
