Stress Physiology and Virulence Characterization of Phomopsis asparagi (Sacc.) Bubák isolated from Asparagus in Jiangxi Province of China

江西省芦笋茎枯病菌的逆境生理响应及致病性分析(英文)

Fungal pathogen of asparagus stem blight was isolated. No significant genetic difference was detected among the three strains with 492 bp long ITS1-5.8S-ITS2 sequence. It was then identified through colony growth, conidia morphology, and molecular characterization. The physiological response to oxidation and osmosis stress, and virulence to Asparagus officinalis L. were analyzed. The results showed that the pathogen causing asparagus stem blight for A. officinalis L. in Jiangxi Province is Phomopsis asparagri (Sacc.) Bubák. Under pure culture conditions, the conidia were oval-shaped (α-type), with colorless single spore and single nucleus, containing 0-2 oil balls. Its vegetative growth rate was higher when cultured on 0.2 × potato dextrose agar (0.2 × PDA) medium than that on oatmeal agar (OA) medium. However, the pycnidia appeared earlier on OA medium than on 0.2 earlier PDA medium. The vegetative growth rate was depressed under oxidation (H2O2) or osmosis (NaCl) stress conditions, and totally inhibited under 7 mmol/L H2O2 or 2.4 mol/L NaCl. All the strains caused typical pathogenic symptoms to Asparagus officinalis L. at 7 days-post-inoculation (dpi) with conidia.

本研究在江西省分离、纯化了3株芦笋茎枯病菌,ITS1-5.8S-ITS2分子分析表明其序列长度均为492bp,ITS区域遗传差异不显著。病原菌菌落生长、分生孢子形态、氧化和渗透逆境胁迫响应及致病性等方面的综合鉴定结果表明:引起江西省芦笋茎枯病的病原为拟茎点霉属天门冬拟茎点霉。在纯培养条件下,其分生孢子呈椭圆形(α型)、无色单孢、单核、内含0-2个油球。病原菌在马铃薯培养基(0.2×PDA)上的营养生长速率高于燕麦培养基(OA),但马铃薯培养基则更易出现分生孢子器。在氧化((H2O2)或渗透(NaCl)胁迫下,菌株表现出营养生长速率降低,且当H2O2或NaCl浓度分别高于7mmol/L或2.4mol/L时,菌丝停止生长。在利用分生孢子接种7d后,菌株均能导致芦笋嫩茎出现典型性感病症状。