RP-HPLC法测定不同厂家阿胶中核苷类化合物的含量

Content Determination of Nucleosides Compound in Equus asinus Products from Different Manufacturers by RP-HPLC

目的:建立同时测定阿胶产品中多个核苷类化合物含量的方法,并评价相关阿胶产品的质量均一性。方法:采用反相高效液相色谱法。色谱柱为Zorbax SB-C18柱,流动相为1mmol.L-1乙酸铵水溶液(A)-甲醇(B)(梯度洗脱),检测波长为260nm,流速为1mL.min-1,进样量为1μL,柱温为30℃。结果:胞嘧啶、胞苷、尿嘧啶、次黄嘌呤、尿苷、鸟苷、腺苷检测浓度分别在一定的范围内与峰面积积分值呈良好的线性关系(均r=0.9999);平均回收率在94.3%～103.8%之间,RSD在0.2%～3.2%(n=3)之间。自制10批阿胶样品的核苷类化合物总含量的RSD为9.1%,而所有检测阿胶样品中核苷类化合物总含量的RSD则高达42.9%。结论:不同厂家阿胶产品质量可能存在较大的差异性;该方法可为阿胶产品的质量控制提供参考。

OBJECTIVE： To develop the method for the simultaneous determination of several nucleosides compounds in Equus asinus products, and to evaluate the quality homogeneity of it. METHODS： RP-HPLC method was adopted. A Zorbax SB C18 column was used for the separation with 1 mmol.L-1 ammonium acetate water solution （A）-methanol （B） as mobile phase （gra- dient elution） at the flow rate of 1 mL.min-1. The injection volume was 1 μL and the column temperature was 30 ℃, and the de- tection wavelength was set at 260 nm. RESULTS： There were good linear relationships between cytosine, cytidine, uracil, hypo- xanthine, uridine, inosine, guanosine and peak area （r=0.999 9）. The recovery rates were 94.3% -103.8%, and RSDs were 0.2% -3.2% （n=3）. The results of determination show that the RSD of total contents of nucleosides compounds for 10 batches self-prepared samples is 9.1%. However, the RSD of total contents of nucleosides compounds for all tested E. asinus samples is 42.9%. CONCLUSION： The quality of E. asinus products from different manufacturers may have great differences. The method can provide reference for the quality control of E. asinus products.