MCP-1、RANTES在慢性移植肾失功肾组织中的表达及意义

目的 探讨单核细胞趋化蛋白-1（MCP-1）和RANTES在慢性移植肾失功（CRAD）患者移植肾组织中的表达及意义.方法 用免疫组织化学技术和计算机真彩色图像分析系统半定量检测32例慢性移植肾失功患者移植肾组织中MCP-1和RANTES的表达,分析与移植肾间质纤维化/小管萎缩程度及炎性细胞浸润程度之间的关系.结果 慢性移植肾失功患者的移植肾组织中MCP-1和RANTES的表达较正常肾组织中明显增加,并随着间质纤维化/小管萎缩及炎症细胞浸润程度而递增.结论 移植肾组织中MCP-1和RANTES的表达升高与慢性移植肾失功的进展有关.

Objective To investige the expression of monocyte chemoattractant protein 1 （ MCP-1 ） and RANTES and their significance in the pathogenesis of chronic renal allograft dysfunction.Method Immunohistochemical assay and computer- assisted genuine colored image analysis system were used to detect the expression of MCP-1 and RANTES in the renal allografls of patients with CARD.The relationship between expression level of mcp-land Rantes and either the grade of inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft was analyzed.Six specimens of healthy renal tissue were used as controls.Results The expressions levels of MCP-l and RANTES were significantly higher in the renal tissues of the patients, compared to normal renal tissues, and the expressions tended to increase with the pathological grades of either inflammatory cell infiltration or interstitial fibrosis/tubular atrophy in renal allograft tissue.Conclusion The up-regulated expression of MCP-1 and Rantes in transplant kidney tissue may have the relationship wih the progressive of the chronic renal allograft dysfunction