基于冻融辅助超声波法的小球藻多糖提取工艺优化

Process optimization of polysaccharides extraction from Chlorella pyrenoidosa based on ultrasound assisted by freezing and thawing

为了优化水提法小球藻多糖提取的工艺,该文在单因素试验的基础上,选择水料质量比、超声波功率、提取时间、冻融-超声波次数进行4因素3水平的正交试验提取小球藻多糖,采用三氯乙酸法去除游离蛋白质。结果表明:小球藻多糖最佳提取条件为水料质量比15,超声波功率600W,超声波作用时间6min和冻融-超声波2次,小球藻粗多糖得率为5.918%。三氯乙酸法脱游离蛋白质以pH值为4为最佳,多糖回收率为57.84%。该研究可为从小球藻中大规模分离和提取以及纯化多糖提供参考。

In order to optimize the technology for the water extraction of polysaccharides from Chlorella pyrenoidosa, orthogonal test considering four factors including the ratio of water to raw material, ultrasonic power, ultrasonic time and extraction times were conducted. Free proteins were removed by TCA method. The results showed that the optimum extraction conditions were obtained as follows： the ratio of water to raw material was 15, extracting temperature was 600W, extracting time was 6 min and extracting times was 2, which led to estimated values of maximal yield of polysaccharides of 5.918%. Conditions of free proteins removed by TCA method was pH4 and the recovery rate of purified polysaccharides was up to 57.84%, which provide an industrial reference for developing new processes of polysaccharides production from Chlorellapyrenoidosa