同型半胱氨酸对气道平滑肌细胞及成纤维细胞的影响

The effect of homocysteine on airway smooth muscle cells and fibroblasts

目的：探讨同型半胱氨酸（ＨＣＹ）对气道平滑肌细胞及成纤维细胞的增殖和成纤维细胞胶原生成的影响。方法：在培养的兔气道平滑肌细胞和大鼠成纤维细胞上，用［３Ｈ］－胸腺嘧啶和［３Ｈ］－脯氨酸掺入方法观察不同浓度 ＨＣＹ对细胞增殖和成纤维细胞胶原生成的影响。结果：ＨＣＹ（０．ｌ～１．０ ｍｍｏｌ／Ｌ）呈浓度依赖性刺激气道平滑肌细胞和成纤维细胞增殖，并呈浓度依赖性刺激气道成纤维细胞的胶原合成与分泌。蛋白激酶Ｃ抑制剂Ｈ７及多粘菌素Ｂ可抑制ＨＣＹ诱导的气道平滑肌细胞增殖。结论：ＨＣＹ刺激气道平滑肌细胞及成纤维细胞增殖，并促进成纤维细胞的胶原合成和分泌。ＨＣＹ刺激气道平滑肌细胞增殖可能通过ＰＫＣ信号传导途径。

AIM: To study the effect of homocysteine (HCY) on proliferation of airway smooth muscle cells and fibroblasts and the effect of HCY on collagen prodution of airway fibroblasts. METHODS: [3H]-TdR incorpora- tion was measured in cultured airway smooth muscle cells. The [3H]-TdR and [3H]-proline incorporation were mea- sured in cultured airway fibroblasts. RESULTS: HCY induced proliferation of airway smooth muscle cells and fibroblasts in a concentration - dependent manner. HCY also induced collagen production of airway fibroblasts in a concentration - dependent manner. The inhibitors of protein kinase C, H7 and polymyxin B, inhibited HCY - induced proliferation of airway smooth muscle cells. CONCLUSIONS: HCY induced proliferation of airway smooth muscle cells and fibroblasts, HCY also induced collagen production of airway fibroblasts. The HCY - induced proliferation of airway smooth muscle cells may be related to the pathway of PKC signal transduction.