苯丙-甲硫-精-苯丙氨酸多肽对豚鼠心室肌细胞Na^+/Ca^(2+)交换尾电流的影响

Effect of Phe-Met-Arg-Phe-NH_2-related peptides on the Na^+/Ca^(2+) exchange tail current in guinea pig ventricular myocytes

目的 研究苯丙 -甲硫 -精 -苯丙氨酸 (FMRFa)多肽对心肌细胞膜钙瞬变触发的 Na+ / Ca2 + 交换尾电流的影响。方法 采用蛋白酶 E急性分离的豚鼠心室肌细胞。利用全细胞膜片钳技术观察 FMRFa多肽对心肌细胞膜 Na+ / Ca2 + 交换尾电流的作用。结果 FMRFa多肽 1、5、10、2 0μm可分别抑制 Na+ / Ca2 + 交换尾电流(13± 3) % ,(2 5± 7) % ,(73± 9) %和 (110± 10 ) %。结论 FMRFa多肽可剂量依赖性地抑制豚鼠心室肌细胞膜钙瞬变触发的 Na+ / Ca2

Objective To study effect of Phe Met Arg Phe NH 2(FMRFa) related peptides on Na +/Ca 2+ exchange tail current in single guinea pig ventricular myocytes;Method In isolated guinea pig ventricular myocytes,effect of FMRFa related peptides on the Na +/Ca 2+ exchange tail current was observed by the whole cell patch clamp technique.Result FMRFa related peptides 1、5、10、20 μm,inhibited Na +/Ca 2+ exchange tail current by (13±3)%,(25±7)%,(73±9)% and (110±10)%,respectively.Conclusion FMRFa related peptides can dose dependently inhibit Na +/Ca 2+ exchange tail current induced by calcium transient.