DEHP介导PPARs对雌性小鼠卵巢功能影响

DEHP mediated by peroxisome proliferator-receptors affect functions of ovary of female mice

目的通过介导过氧化物酶体增殖剂激活受体(PPARs)探讨邻苯二甲酸二乙基己酯(DEHP)对雌性生殖系统影响。方法将40只21 d雌性ICR小鼠随机分成DEHP 0、20、100、500 mg/kg剂量组,连续染毒10 d,停止染毒24 h后处死动物,用实时聚合酶链式反应(real time-PCR)技术测定小鼠PPARs mRNA表达水平,用酶联免疫吸附法(ELISA)检测小鼠血清中雌二醇、孕酮水平。结果 100、500 mg/kg DEHP组卵巢湿重分别为(0.01±0.003)、(0.01±0.007)g,脏器系数分别为(0.13±0.02)%、(0.12±0.03)%,与对照组比较,明显降低(P<0.05);500 mg/kg DEHP组小鼠血清中雌二醇水平明显低于对照组(P<0.05);各染毒组小鼠血清孕酮水平与对照组之间无明显差异(P>0.05);与对照组比较,各染毒组PPARα、PPARβmRNA表达水平无明显变化(P>0.05),PPARγmRNA表达水平明显升高(P<0.05)。结论 DEHP作为一种过氧化物酶体增殖剂可以通过改变PPARs基因表达水平影响雌性小鼠卵巢功能。

Objective To assess the effects of di-2-ethylhexyl phthalate（DEHP） on female reproductive system in mice mediated through peroxisome proliferator-receptors（PPARs）.Methods Fourty female ICR mice of 21 days were randomly divided into 0,20,100,and 500 mg / kg dose group.With continuous DEHP exposure for 10 days and 24 hours after cessation of the exposure,the mice were killed.PPARs mRNA expression levels were determined by real time-PCR.Estradiol and progesterone levels in serum were detected with enzyme-linked immunosorbent assay（ELISA）.Results The ovarian wet weight and organ coefficient decreased significantly in 100 and 500 mg/ kg DEHP exposure groups compared with those of the control group（P〈0.05）.Serum estradiol level in 500 mg/ kg DEHP group decreased significantly compared with the control group（P〈0.05）.Compared with that of the control group,progesterone level showed a significant difference（P〉 0.05）.The PPARα,PPARβ mRNA expression levels in DEHP exposure groups were not significant different from those of the control group（P〉 0.05）.PPARγ mRNA expression level increased significantly in DEHP expouse groups compared with the control group（P〈0.05）.Conclusion As a peroxisome proliferator mediating PPARs gene expression,DEHP could affect ovarian function in mice.