CatWalk步态分析用于大鼠切口痛模型的评估

CATWALK GAIT ANALYSIS IN INCISIONAL PAIN MODEL IN RATS

目的:采用CatWalk步态分析仪对切口痛大鼠的步态变化进行分析,并将步态参数与vonFrey丝测定的机械缩足阈值(mechanical withdrawal threshold,MWT)进行比较,研究其相关性。方法:采用Brennan法建立大鼠切口痛模型。分别于术前24 h、术后2 h、24 h、48 h、72 h,用CatWalk步态分析仪及von Frey丝进行相关步态参数和机械缩足阈值的测定。对测定结果进行统计分析。结果:术后大鼠术侧脚印面积变小、站立时相持续时间缩短、举步时相持续时间延长,举步速度减慢。脚印面积、站立时相持续时间、举步时相持续时间、举步速度与机械缩足阈值的相关性具有统计学意义。结论:CatWalk是一种评估切口痛模型早期机械性痛觉超敏的新方法。

Objective： To compare the gait parameters recorded on the CatWalk with the mechanical withdrawal threshold （MWT） tested with von Frey hair in incisional pain model in rats, and to evaluate the correlation between the CatWalk parameters and MWT. Methods： The incisional pain model was adopted as described by Brennan. All rats were tested with von Frey hair and the CatWalk at 24 h before incision and 2 h, 24 h, 48 h and 72 h after incision. Results： Compared with the baseline before incision and healthy side, print area, stance phase and swing speed of the injured paw decreased significantly, and swing phase of the injured paw increased significantly after incision. Moreover, these CatWalk parameters showed a statistical correlation with MWT. Conclusion： The CatWalk gait analysis is a novel tool to evaluate early mechanical allodynia in incision pain model.