RT-PCR检测自然流产妇女外周血淋巴细胞中柯萨奇B组病毒核酸

Detection of Coxsackievirus B RNA in Lymphocyte of Women with Miscarriage by RT-PCR

目的 :通过对 40例自然流产妇女和 40例人工流产妇女的外周血淋巴细胞柯萨奇B组病毒 (CVB)核酸的检测 ,研究CVB感染与自然流产可能关系。方法 :用CVB特异性引物进行逆转录聚合酶链反应 (RTPCR)检测外周血淋巴细胞中病毒核酸。结果 :自然流产妇女外周血淋巴细胞中CVB检出率为 85 % (34/ 40 ) ,人工流产妇女检出率为 37.5 % (15 / 40 ) ,两者比较差异有显著性 (P <0 .0 1)。结论

Objective: To study the relationship between coxsackievirus B infection and miscarriage. Methods: 40 lymphocyte samples from women with miscarriage and 40 lymphocyte samples from women with induced abortion were carrtied out reverse_transcription polymerase chain reaction(RTPCR) to detect coxsackievirus group B RNA. Results: 34 in 40 samples from miscarriage group were positive, only 15 in 40 samples from induced abortion group were positive. There is a statistically significant difference(P<0.01).Conclusion: Coxsackievirus B may play a role in miscarriage.