摘要
目的 :探讨P5 3和nM 2 3基因表达在人非小细胞肺癌中的意义。方法 :收集本院 47例非小细胞肺癌根治标本的石蜡包埋组织。微波修复抗原、SP免疫组织化学方法染色 ,光镜观察。结果 :47例中 ,低分化癌组P5 3蛋白阳性率 88.88% ,高分化癌组P5 3蛋白阳性率 44 % ,差异有高度显著性 (P <0 .0 1)。有肺门纵隔淋巴结转移组的P5 3蛋白阳性率 82 .6 0 % ,无淋巴结转移组的P5 3蛋白阳性率 2 5 .0 0 %。差异有显著性 (P <0 .0 5 )。P5 3蛋白在肺腺癌与肺鳞癌中的表达差异无显著性 (P >0 .0 5 )。 47例中 ,肺鳞癌有肺门纵隔淋巴结转移组nM2 3阳性率2 1.42 % ;无淋巴结转移组nM 2 3阳性率 6 2 .5 0 % ,差异有高度显著性 (P <0 .0 1)。nM2 3在肺鳞癌与肺腺癌 ,在肺腺癌中有无淋巴结转移组表达及在非小细胞肺癌高、中、低 3种不同分化组表达差异均无显著性 (P >0 .0 5 )。结论 :P5 3蛋白表达程度与非小细胞肺癌的分化程度及淋巴结转移有关 ,而nM2 3能抑制肺鳞癌的转移。
Objective:To study the significance of the gene express of P53 and nM23 in human non-small cell carconoma in lung.Methods:47 operated cases of paraffin embeded tissues of human non-small cell carcimona in lung were collected.immunohistochemical S-P method was used.It is found that (in low differentiation group),the positive rate of P53 was 88.88%,while that of P53 in high differentiation was 44% (P<0.05).The positive rate of P53 in lymph node metastasis was 82.6%,while that of no lymph node metastasis was 25%(P<0.05).The postive rate of nM23 lung's sequamous cells cancer with lymph node metastasis was 21.42% while that of no lymph node metastasis was 62.50%(P<0.01).Conclusion:the expression of P53 was related with the differentiation and lymph node metastasis,which showed that the expression of P53 was relatied with the progress and malighant degree.While the expression of nM23 was higher in lung sequamous cell cancer with no lymph node metastases than that of lymph node matastasis,which showed nM23 protein could prohibit the metastasis of lung sequamous cell cancers.
