摘要
目的 :探讨血管紧张素Ⅱ_1型受体 (AT1R)基因A116 6 /C多态性与国人原发性高血压及其合并冠心病的关系 ,并探讨原发性高血压的发病机制。方法 :应用聚合酶链反应、限制性内切酶酶解的方法检测 70例健康人和 70例高血压患者 ,其中 34例合并冠心病患者的AT1R基因型。结果 :原发性高血压组的C等位基因频率 13.6 % ,显著高于正常对照组的 3.6 % (P <0 .0 0 5 ) ;高血压合并冠心病组的C等位基因频率 13.2 % ,与高血压无合并症组的 13.9%差别无显著性意义 (P >0 .0 5 )。结论 :提示AT1R基因A116 6 /C多态性是原发性高血压的重要遗传因素。
Objective:To identify the polymorphism of angiotensin Ⅱ types 1 receptor gene in Chinese essential hypertension.Methods:This study included 70 hypertensive (involved 34 hypertensives complicated with coronary artery disease) and 70 normotensive subjects.AT1R genotyping was analyzed by polymerase chain reaction,digestion of restriction enzyme and electrophoresis.Results:The frequencies of C allele among the essential hypertension group (12.9%) were higher than those among the control group (3.6%,P<0.005).The frequencies of C allele were no difference between hypertensives complicated with coronary artery disease and hypertensives without cardiovascular or cerebrovascular disases.Conclusion:The AT1R gene A1166/C polymorphism is probably an important hereditary factor in Chinese essential hypertension.
