异氟烷预处理对脑缺血再灌注大脑保护作用的实验研究

Protective Effects of Isoflurane on Cerebral Injury from Cerebral Ischemia-reperfusion in Rats

目的:探讨全身麻醉药异氟烷对大鼠脑缺血再灌注后脑损伤的保护作用。方法:随机将20只SD大鼠分为2组,每组10只。氯胺酮(1mg/kg)分别对两组动物施行基础麻醉。对照组进行假手术;吸入组应用异氟烷持续吸入维持麻醉后进行手术,大脑缺血再灌注后取脑组织标本。测定脑组织NF-κB的含量以及脑组织形态学观察。结果:异氟烷对实验动物维持麻醉,可以有效地降低脑组织中NF-κB的含量,并且组织损伤性变化明显减轻。结论:异氟烷对大鼠脑缺血再灌注脑组织损伤具有明显的保护作用。

Objective： To study the protective effects of isoflurane on cerebral injury from cerebral ischemia-reperfusion in rats.Methods： 20 rats were randomly divided into two groups with 10 rats each group.The two groups were performed basic anesthesia with ketamine（1mg/kg）.Direct surgery for control group,while experimental group were inhalationally anesthetized with isoflurane to maintain anesthesia during surgery at the same time.In two groups after cerebral ischemia-reperfusion,cerebral tissues were sampled.Observed morphological changes of the lung tissues and determined NF-κB in cerebral tissue.Results： Isoflurane anesthesia during cerebral ischemia-reperfusion in rats was effective in reducing NF-κB in cerebral tissue,and cerebral tissue damage became attenuated.Conclusion： Isoflurane was effective to cerebral injury from cerebral ischemia-reperfusion in rats.