肝细胞肝癌中脆性组氨酸三联体和p21蛋白的表达及意义

The expression and clinical significance of FHIT protein and p21 protein in liver carcinoma

目的:探讨肝细胞癌(HCC)组织中脆性组氨酸三联体(FHIT)蛋白和p21蛋白的表达及临床意义。方法:用Western blot方法检测36例HCC、32例癌旁和14例正常肝组织中FHIT蛋白和p21蛋白的表达,并结合临床病理指标进行分析。结果:FHIT蛋白在HCC中的表达(0.934±0.622)低于癌旁(2.298±1.050)和正常对照组(2.457±0.958),差异有统计学意义(P<0.01);p21蛋白在HCC中的表达(2.604±0.316)高于癌旁(1.241±0.547)和正常对照组(1.056±0.186),差异有统计学意义(P<0.05)。在高、中、低分化HCC中FHIT蛋白表达逐渐减低;而p21蛋白在上述组织中表达逐渐增强,差异均有统计学意义(P<0.05)。门静脉癌栓组中FHIT表达低于无门静脉癌栓组;p21在门静脉癌栓组中表达增高,差异有统计学意义(P<0.05)。在HCC中FHIT和p21的表达呈负相关(r=-0.329,P=0.027)。结论:FHIT的低表达与p21高表达和HCC的病理分型和侵袭转移有关,FHIT基因的突变或缺失可能通过上调p21基因的转录而导致恶性肿瘤的发生。

Objective：To explore the expression and clinical significance of fragile histidine triad,（FHIT） protein and p21 protein in hepatocellular carcinoma（HCC） tissues.Methods：The expression of FHIT protein and p21 protein in 36 of HCC,32 paracarcinoma tissues and 14 normal liver tissues was detected by Western blot,and the result was analyzed combine the clincopathlogical indices.Results：The expression of FHIT protein in HCC（0.934±0.622）was lower than that in paracarcinoma tissues（2.298±1.050）and normal comparison（2.457±0.958）（P〈0.01）;The expression of p21 protein in HCC（2.604±0.316）was higher than that in paracarcinoma tissues（1.241±0.547）and normal comparison（1.056±0.186）（P〈0.05）.The expression of FHIT protein in high differentiation,moderate differentiation and low differentiation was gradually decreased;The expression of p21 protein in above-mentioned tissue was gradually increased.The difference all had statistical significance（P〈0.05）.The expression of FHIT protein with carcinoma embolus of portal vein is obviously lower than those without carcinoma embolus of portal vein;The expression of p21 protein in above-mentioned tissue was enhancement,the difference had statistical significance（P〈0.05）.Negative correlation was found between the positive expression rate of FHIT protein and p21 protein（r=-0.329,P=0.027）.Conclusion：The low expression of FHIT protein and high expression of p21 protein was associated with pathological type,infestation and metastasis.The catastrophe or absence of FHIT protein lead to the happening of malignant tumour through up-regulation the transcription of gene p21.