摘要
目的:观察胃癌患者外周血及癌组织中上皮细胞黏附分子(EP-CAM)及存活素的表达水平及探讨其在胃癌发生及发展中的临床意义。方法:采用RT-PCR检测正常对照组和胃癌组患者外周血EP-CAM及存活素mRNA水平;免疫组化法观察癌旁上皮组织和胃癌组织中EP-CAM及存活素蛋白表达。结果:正常对照组外周血EP-CAMmRNA相对表达水平为0.05±0.01,存活素mRNA相对表达水平为0.02±0.01,胃癌组EP-CAM相对表达水平为0.82±0.02,存活素相对表达水平为0.61±0.04,两者均显著高于正常对照组(P<0.01);癌旁上皮组织中EP-CAMMOD为0.005±0.001,存活素MOD为0.004±0.001,胃癌细胞EP-CAM的MOD为0.309±0.054,存活素MOD为0.331±0.066,两者均显著高于癌旁对照组织(P<0.01)。结论:EP-CAM及存活素mRNA和蛋白高表达可能参与了胃癌的发生发展。
Objective: To investigate the expression of EP-CAM and survivin in peripheral blood and tumor tissue of human gastric carcinoma and their relationship with the genesis and development of gastric carcinoma. Method: RT-PCR was used to examine the levels of EP-CAM and survivin mRNA in the peripheral blood in gastric carcinoma and normal control. Immunohistochemistry was used to examine the expressions of EP-CAM and survivin proteins in gastric carcinoma and normal gastric mucosa. Results: Compared with the control group, the levels of EP-CAM and survivin mRNA in peripheral bolld of gastric cancer group were 0.82±0.02 and 0.61±0.04, the expressions of EP-CAM and survivin proteins in tumor tissue of gastric cancer group were 0.309±0.054 and 0.331±0.066, respectively. They were significantly higher than normal control (P<0.01). Conclusion: The effect of EP-CAM and survivin up-regulating is correlated to development of gastric cancer.
出处
《微循环学杂志》
2012年第3期21-23,76,共4页
Chinese Journal of Microcirculation
基金
武汉市卫生局临床重点学科研究项目(2006)
