肠缺血-再灌流大鼠小肠血管内皮细胞ICAM-1表达的变化及其与肠损伤的关系

The relationship between the ICAM 1 expression of hemangioendotheliocyte and the dysfunction of murine small bowel in an intestinal ischemia reperfusion model

目的 探讨肠缺血 再灌流过程中肠局部血管内皮细胞的细胞间粘附分子 1(intercellu laradhesionmolecule 1,ICAM 1)mRNA和蛋白表达的变化与中性粒细胞聚集的关系及其在肠损伤中的作用。方法 采用肠系膜上动脉夹闭的方法制作大鼠肠缺血 再灌流模型 ,应用逆转录 聚合酶链反应及免疫组织化学的方法观察小肠ICAM 1表达的变化 ,同时检测组织髓过氧化物酶活性及反映肠功能的指标D 乳酸、二胺氧化酶活性的变化。结果 肠缺血 0 5～ 1 5h ,小肠ICAM 1mRNA表达增加 ,免疫组织化学显示其蛋白表达在肠缺血 1h及再灌流 1h时增多 ;肠组织髓过氧化物酶活性同时升高 (P <0 0 5 ) ,并持续至再灌流后 6h ;血浆二胺氧化酶活性在肠缺血 1h和再灌流后 2h升高 (P<0 0 5 ) ,血浆D 乳酸浓度在肠缺血 1 5h时开始增加 ,再灌流后 0 5h和 1h时大幅度升高 (P <0 0 1,P <0 0 5 )。结论 肠缺血 再灌流时肠血管内皮细胞ICAM 1表达增加 ,由此介导的中性粒细胞在局部聚集。

Objective[WT5”BZ] To investigate the change in ICAM 1 expression on gut vascular endothelial cells during ischemia reperfusion, and its influence on polymorphonuclear (PMN) aggregation and gut dysfunction in a murine model.[WT5”HZ] Methods[WT5”BZ] The mRNA transcription and protein translation levels of ICAM 1 were assayed with RT PCR and immunohistochemistry in an ischemia reperfusion rat model, and gut myeloperoxidase (MPO) activity, plasma D lactate concentration and diamine oxidase (DAO) level were determined as well. [WT5”HZ]Results[WT5”BZ] The ICAM 1 mRNA expression increased at 0 5～1 5 h post ischemia and its protein highly expressed at 1 h post ischemia and 1 h reperfusion; gut MPO activity was enhanced( P <0 05) in accordance with ICAM 1 and lasted for 6 h; plasma DAO activity was increased from 1 h ischemia to 2 h post reperfusion( P <0 05); plasma D lactate concentration peaked at 0 5～1 h post reperfusion( P <0 01, P <0 05). [WT5”HZ]Conclusion[WT5”BZ] The aggregation and activation of PMN in intestinal tissue is associated with the high expression of ICAM 1 on intestinal vascular endothelial cells in gut ischemia reperfusion rats, which might be the pathophysiological mechanisms underlying intestinal mucosa injury and enhanced intestinal permeability. [WT5”HZ]