河南鸡源沙门菌的分离鉴定及其耐药性分析

Isolation and identification of Salmonella from chickens in Henan and drug resistance

对来自河南郑州、许昌、周口等20个地市101个鸡场的161份疑似沙门菌感染的病料进行病原菌分离鉴定和耐药性试验。通过细菌分离培养、形态染色、生化试验、血清学试验、致病性试验,结果分离鉴定出33株沙门菌,且均具有明显的致病性。药敏试验发现,33株分离株除对头孢曲松较为敏感(51.5%)外,对其他13种药物均呈不同程度耐药性,对复方新诺明的耐药率最高,为81.8%(27/33),其次为喹诺酮类药物,且表现为交叉耐药现象。对环丙沙星产生耐药性的菌株占分离株总数的75.7%(25/33),对诺氟沙星耐药的菌株占69.7%(23/33),对氧氟沙星耐药的菌株占63.6%(21/33)。结果表明:不同地区的分离菌株对各种药物的耐药性不同,同一地区的分离菌株对各种药物的耐药性也存在显著差异。

One hundred and sixty-one samples from 101 chicken farms in Henan were collected for isolation and identification of Salmonellaand surveillance of drug resistance. Thirty-three isolates were identified as Salmonella based on culture in well-established media, staining and biochemical tests. And the isolates were demonstrated to be virulent. The drug sensitivity test showed that 33 isolates were resistant to 14 kinds of drugs except for ceftriaxone with the sensitivity of 51.5%. The cotrimoxazole resistance rate was the highest and up to 81.8% （27/ 33） , followed by quinolones. The resistance rates of ciprofloxacin, norfloxacin and ofloxacin were 5.7% （25/33） , 69.7% （23/33） and 63.6% （21/53）, respectively. The results indicated that significant differences in antibiotic resistance were found in the strains from the different areas, and the strains from the same area have also developed different antibiotic resistances.