桑叶药材水浸出物和指标成份的含量测定研究

目的研究桑叶药材的质量,通过药材提取物(流膏、干膏和浸出物)和指标成份含量的测定以及药材的薄层鉴别,从定量角度来研究药材质量。方法主要采用高效液相色谱法,十八烷基硅烷键合硅胶为填充剂,甲醇-乙腈-醋酸-水(15:10:2.6:72.4)为流动相,检测波长为358nm,流速1mL/min,柱温30℃。结果不同产地5批桑叶原药材、流浸膏和干膏各样品之间的芦丁含量具有明显的差异,即便是同一产地不同批号的样品芦丁含量也存在很大的差别。其原药材、流浸膏和干膏芦丁含量的大小次序是一致的,广西090901的含量最高分别是0.30%、0.124%和0.048%,湖北2最低分别是0.11%、0.095%和0.035%。浸出物的含量测定亦有差异,但均符合标准。水分测定几乎无差异符合规定。结论通过对桑叶药材浸出物和指标成份的含量测定,可从定量的角度更好地对药材质量进行控制。

Objective To study the quality of medicinal herbs of Mulberry Leaf. The quality of medicinal herbs is studied quantitatively by determining the content of extracts and component indices of Mulberry Leaf medicinal herbs and TLC of the herbs. Methods The chromatographic separation was achieved on a Diamonsil ODS-C 18 column with amobile phase of methanol-acetonitrile-aceticacid-water solution（15：10：2.6：72.4）. The flow rate was 1.0 mL.mint and the column temperature was 30℃. Results The differences can be seen obviously among 5 batches of sample of rntin content in origin mulberry leaf raw herbs, flow paste and dry extract from different places. Additionally, though the samples of rutin content of different batches from the same origin, there is also a big difference. The content of Guangxi 090901 maximum is 0.30%, 0.124% and 0.048%, and Hubei 2 lowest is 0.11%, 0.095% and 0.035%, respectively. Conclusion According to determination and analyses of the content of extracts and component indices of Mulberry Leaf medicinal herbs, the quality of medicinal herbs would be controlled better from a quantitative point of view.