苗药双肾草多糖含量测定及其免疫调节作用研究

Determination of Polysaccharides in Herminium bulleyi and Its Immunoloregulation Effects

[目的]测定苗药双肾草中多糖的含量并研究其免疫调节作用。[方法]以葡萄糖为对照品,采用苯酚-硫酸法测定双肾草多糖的含量,并研究双肾草多糖对小鼠的免疫调节作用。[结果]双肾草多糖含量为58.5%;双肾草多糖可明显增加小鼠的脾脏重量,促进抗体的产生,对正常小鼠体液免疫具有一定的增强作用。[结论]双肾草多糖对机体的的特异性免疫功能有一定的促进作用。

[Objective] To detect the polysaccharides content in Herminium bulleyi（Rolfe） Tang et Wang and its immunomodulatory effects.[Method] With glucose as the control,polysaccharides content in H.bulleyi was detected by phenol-sulphuric acid method.And the immunomodulatory effects of H.bulleyi polysaccharides on mice were researched.[Conclusion] Content of H.bulleyi polysaccharides was 58.5%,which could significantly enhance the spleen weight of mice,promote the development of antibody,and enhance the humoral immunity of mice.[Conclusion] H.bulleyi polysaccharides had certain promotion effects on the specific immunity of organism.