四川安岳柑橘衰退病病原鉴定及序列分析

Molecular Identification and Sequence Analysis of the Pathogen Causing Citrus Tristeza Disease in Anyue County of Sichuan Province

[目的]探明引起四川安岳柑橘衰退病的病原。[方法]利用RT-PCR、克隆、测序等技术对四川安岳地区的柑橘类果树进行柑橘衰退病毒(CTV)检测,并对其外壳蛋白(CP)全序列进行了分析。[结果]81个疑似CTV侵染的样品中共有18个检测呈阳性,检出率为22.2%,表明安岳地区柑橘类果树衰退病由CTV引起。基于CP全序列同源性比较及进化分析表明,危害该地区柑橘类果树的CTV分属5个组群,亲缘关系较复杂。[结论]为安岳地区CTV的防控与防治提供了理论依据。

[Objective] The aim was to identify the pathogen to cause citrus tristeza disease in Anyue County of Sichuan Province.[Method] The complete nucleotide sequence of citrus tristeza virus（CTV） coat protein（CP） was determined by using RT-PCR,cloning and sequencing techniques.[Result] Eighteen CTV isolates（22.2%） were collected in a total of 81 field citrus samples in Anyue,Sichuan province.Sequence comparison and phylogenetic tree of 18 CTV isolates and other CTV isolates indicated that citrus tristeza disease in Anyue was caused by CTV.These diseases belonged to five groups,and the genetic relationship between CTV was complicated.[Conclusion] The research provides theoretical basis for the prevention and control of CTV in Anyue area.