摘要
In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0 1—10 ng/mL. [WT5HZ]
In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0 1—10 ng/mL. [WT5HZ]
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2000年第6期870-872,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 !(批准号 :2 9775 0 0 2 )
关键词
水凝胶
毛细管电泳
免疫分析
雌二醇
NIPAAM
Hydrogel
Capillary electrophoresis
Laser induced fluorescence
Immunoassay
Estradiol
