miRlet-7b抑制乳腺癌MCF-7细胞迁移及其机制

Mechanism of miR let-7b inhibiting breast cancer MCF-7 cells migration

目的:观察miR let-7b对乳腺癌MCF-7细胞迁移能力的影响并探讨其作用机制。方法:合成miR let-7b并转染乳腺癌MCF-7细胞,以miR-control作参照。通过划痕实验观察转染前后细胞迁移能力的变化,运用PCR和蛋白质印迹法检测转染miR let-7b前后MCF-7细胞内IL-6表达水平的变化。最后通过信息生物学软件及双荧光酶素报告基因方法验证miR let-7b与IL-6结合。结果:与对照组比较,转染miR let-7b的MCF-7细胞迁移能力明显降低,而anti-miR let-7b明显促进细胞迁移。转染miR let-7b的乳腺癌MCF-7细胞中IL-6的表达水平较对照组明显降低,而转染anti-miR let-7b的细胞内IL-6的表达水平明显提高。信息生物学软件和双荧光酶素报告基因证实miR let-7b与IL-6结合。结论:miR let-7b在转录水平抑制乳腺癌MCF-7细胞IL-6的表达从而抑制MCF-7细胞的迁移。

Objective： To observe the effect of miR let-7b on inhibiting breast cancer MCF-7 cells migration and to investigate it′s mechanism.Methods： miR let-7b were composed and transfected into breast cancer MCF-7 cells.MiR-control was taken as a reference.The migration of the cells was tested by scratch test and the IL-6 level of MCF-7 cells before and after transfected was tested by PCR and west-blot.Then the combination of miR let-7b and IL-6 was verification by Bioinformatics software and Dual luciferase.Results： Compared with the control group,miR let-7b made the migrating ability of MCF-7 cells reduced,while the anti-miR let-7b strengthen it.Those breast cancer MCF-7 cells which were transfected by miR let-7b had a lower level of IL-6,while those transfected by anti-miR let-7b had a higher level of IL-6.It was verificated by Bioinformatics software and Dual luciferase that miR let-7b was combined with IL-6.Conclusion： miR let-7b inhibited the expression of IL-6 of breast cancer MCF-7 cells at the genetic level and then inhibited the migration.