金黄色葡萄球菌临床分离株生物膜形成能力及其相关基因的检测

Detection of biofilm formation and biofilm-associated genes of Staphylococcus aureus clinical isolation

目的:检测临床分离金黄色葡萄球菌(金葡菌)的生物膜形成能力及其相关基因。方法:用半定量微量平板法检测临床分离的92株金葡菌生物膜形成能力,并通过扫描电镜观察生物膜形态,用PCR法来检测生物膜形成的相关基因ica和sarA。结果:92株临床分离金葡菌菌株均可形成生物膜,PCR结果显示100%的分离株均含有sarA基因,78株(84.78%)含有ica基因。结论:临床分离的金葡菌都具有形成生物膜的能力,生物膜形成的相关基因ica和sarA的携带率较高。

Objective： To detect biofilm formation and biofilm-associated genes of S.aureus clinical isolation.Methods： The biofilm formation was determined by semi-quantitative microplate assay and scanning electron microscopy method,and both ica and sarA genes were detected by PCR in 92 strains of S.aureus clinical isolation.Results： All of 92 strains in this study could form biofilm,and carry the sarA gene;78 strains（84.78%） carried the ica gene.Conclusion： The S.aureus clinical isolations had the ability of biofilm formation,and the gene of ica and sarA were prevalent in S.aureus.