阿尔茨海默病和血管性痴呆患者的代谢综合征和对氧磷酶-2基因311多态性研究

The study on components of metabolism syndrome and paraoxonase 2-311 polymorphism in Alzheimer disease and vascular dementia

目的探讨阿尔茨海默病(AD)和血管性痴呆(VD)患者间代谢综合征组分与对氧磷酶-2(PON2)311基因多态性的差异。方法研究对象包括49例AD患者和23例VD患者。用聚合酶链反应-限制性片段长度多态性分析法检测患者PON2基因311位点多态性。测量患者体重、身高、腹围、血压,计算体质指数。检测空腹血清胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白、空腹血糖(FBS)等。应用简明智能评定量表(MMSE)、日常生活能力量表(ADL)、Hachinski缺血量表等评定三组患者的认知等临床特征。结果 AD和VD两组间的血压、体质指数、腹围、血清甘油三酯、胆固醇、高密度脂蛋白、低密度脂蛋白、空腹血糖差异无统计学意义(P>0.05)。组间的PON2311基因型C/C、C/S和S/S分布差异无统计学意义,组间C和S等位基因频率也无统计学差异。AD组中C/C型的舒张压高于C/S型;S/S型的血清甘油三酯水平高于C/S型(P<0.05)。VD组中上述三种基因型间血脂、血糖、血压、腹围等无统计学差异。结论 AD和VD患者代谢综合征各项组分、PON2311基因型和等位基因频率均无显著性差异。AD患者PON2311C纯合子可能与较高的舒张压有关;而PON2311S纯合子可能与较高的甘油三酯水平有关。而在VD患者中未发现这种关系。

Objective To investigate the difference of metabolic syndrome and paraoxonase2 （PON-2） 311 polymorphism between Alzheimer＇ s disease （AD） and vascular dementia （VD）. Methods The subjects were 49 patients with AD and 23 patients with VD. The following were collected by the investigators： demographic data, height, weight, waist circumference, blood pressure and et al. Blood sera were used for biochemical analysis including fasting blood glucose, triglyceride, cholesterol, HDL and LDL. The PON-2-311 polymorphism was measured by restriction fragment length polymorphism analysis. Clinical presentation of AD was assessed by MMSE, ADL and Hachinski ischemic scale. Results There were no significant differences in blood pressure, body mass index, waist circumference, fasting blood glucose, serum triglyceride, cholesterol, LDL and HDL level between AD and VD groups （P 〉 0. 05）. No significant differences in genotype distribution or allene frequencies were identified for PON-2-311 between AD and VD group. Diastolic pressure of AD patients with PON-2 C311C was significantly higher than that of patients with PON-2 C311S. Serum triglyceride of AD patients with PON-2 S311S was significantly higher than that of patients with PON-2 C311S （ P 〈 0. 05 ）. No significant differences were observed among three polymorphisms of PON- 2 in patients with VD （P 〉 0. 05 ）. Conclusions Our finding suggests that there were no significant differences in components of metabolic syndrome and genotype distribution or allele frequencies between patients with AD and VD. For AD patients, significant association were observed between the PON-2 C311C genotype and higher level of diastolic pressure,and what＇s more,between the PON-2 S311S genotype and higher serum triglyceride.but not for that of VD.