稻壳纤维的柱层析性能研究

Study on Chromatography Property of Rice Hull Cellulose

试验了稻壳纤维作为柱层析介质的性能 ,并制成亲和层析介质纯化脂肪酶。结果表明稻壳纤维层析过程为化学物理混合吸附过程 ,亲和层析性能良好 ,通过层析可将脂肪酶纯化 2倍 ,回收率达 81 .6%。适合较大规模层析分离。

The proporty of rice hull cellulose as chromatography is tested. The rice hull cellulose is memufacted to affinity chromatography medium to separate lipase. The results show chromatography process of rice hull cdlulose is a chemical and physical mixture adsorption. The property of affinity chromatography is well. The lipase can be purifyed to 2.11 times by chromatography, and the recovery ratio is 81.6 percent. Rice hull cellulose suits to apply larger scale chromatography separation.