期刊文献+

离子对高效液相色谱荧光检测法测定四环素类药物 被引量:6

Determination of Tetracyclines as Al^(3+) Complexes by Ion-pair Reversed-phase Chromatography with Fluorescence Detection
下载PDF
导出
摘要 报道了利用Al3+作为柱前衍生试剂,高效液相色谱分离荧光检测法检测测定四环素类药物的新方法。探讨了四环素类药物-Al3+络合物的衍生条件及其色谱分离条件对荧光强度的影响,3种药物的检出限达70-150pg。 A method is described for the determination of tetracyclines as complexes with Al3+ by reversed-phase high-performance liquid chIDInatogIahy and fluonence detection.The complexes of tetracycline-, chlortetracycline-and minocycline-Al3+ were derivatized in a solution containing buffer of pH=2. 9 and separated on a Kromasil ODS column(250 x 4.6 mm, I. D. 5μm) using acetonitrile-water (90:10, V/V) containing 0.025 mol/L Li2SO4 (pH=2.9) as mobile phase at a flow rate of 0.8 mL/min, and detect at λex = 383 nm andλex = 480 nm.The detection limits were 0.1 ng for tetracycline,0.15 ng for chlortetracycline and 70 pg for minocycline.The method has been applied to the determinations of eye unguent of chlortetracycline and cortison eye unguent of tetracycline with satisfactory results.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2000年第6期745-748,共4页 Chinese Journal of Analytical Chemistry
关键词 四环素类药物 荧光检测 柱前衍生 HPLC Liquid chromatography, tetracyclines,fluorescence detection,aluminum(Ⅲ),derivatization
  • 相关文献

参考文献2

二级参考文献1

  • 1傅承光,第六次全国色谱学术报告会文集.下,1987年

共引文献5

同被引文献142

引证文献6

二级引证文献20

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部