稠环芳烃在烷基膦酸改性锆镁复合氧化物固定相上的反相液相色谱保留行为

Retention Behavior of Some Polycyclic Aromatic Hydrocarbons on Alkylphosphonate Modified Magnesia Zirconia Composite Stationary Phase for Reversed Phase Liquid Chromatography

以稠环芳烃为探针 ,考察了烷基膦酸改性锆镁复合氧化物材料的反相色谱性能。研究了稠环芳烃类化合物的结构与其保留值的关系 ,比较了烷基膦酸改性锆镁复合氧化物固定相和十八烷基键合硅胶 Zorbax ODS对稠环芳烃异构体的选择性 ,并对可能的保留机理进行了讨论。以甲醇 -水 (体积比为 75∶ 2 5)为流动相 ,在烷基膦酸改性锆镁复合氧化物固定相上分离了

An alkylphosphonate modified magnesia zirconia composite stationary phase (PZMS) for reversed phase liquid chromatography was prepared. The reversed phase chromatographic performance of the new packing material was investigated with some polycyclic aromatic hydrocarbons (PAHs) as solutes. The effect of the methanol content in the mobile phase on the retention of PAHs was examined. The logarithmic capacity factor of PAHs was decreased linearly with increasing volume percent of the methanol in the mobile phase, which is similar to that observed on Zorbax ODS. The capacity factor of PAHs on the new stationary phase was also correlated to their partition coefficient between n octanol and water (log P ). A good linear relationship between them was obtained. These results show that the new material can behave as a reversed phase packing. The separations of phenanthrene and anthracene, and terphenyl isomers were investigated on the new stationary phase and the Zorbax ODS, respectively. The retention of the solutes on the Zorbax ODS is greater than that on the new stationary phase, but the separation selectivity of the solutes on the new stationary phase is better than that on the Zorbax ODS. The better separation selectivity on the new stationary phase may be contributed from the Lewis acid base interaction between the solutes and the residual Lewis acid sites on the stationary phase.