反相高效液相色谱法测定肾舒冲剂水煎液中的小檗碱

Determination of Berberine in Decocted Liquid from Shenshu Granules with Water by Reversed\|Phase Liquid Chromatography

:应用反相高效液相色谱法检测了肾舒冲剂水煎液中的小檗碱。样品经超声提取后 ,以 C1 8化学键合硅胶为固定相 ,乙腈 - 0 .0 4 mol/ L H3PO4(体积比为 4 2∶ 58)为流动相 ,用 34 9nm的波长定量检测。测定结果表明 ,小檗碱的质量浓度在 1.2～ 19.2 mg/ L范围内线性良好 ,最低检测限为 0 .6m g/ L,测定批内 ( n=5)及批间 ( n=5)相对标准偏差分别为 0 .6%～ 3.5%和 5.3%～ 6.5% ,回收率为 89.10 %～ 91.35%。

A reversed\|phase liquid chromatographic method has been developed and validated for the analysis of berberine in decocted liquid from Shenshu granules with water. The drug was extracted with supersonic wave and then measured by HPLC using a Hypersil C\-\{18\} column and an acetonitrile \|0 04 mol/L H\-3PO\-4 (42∶58,V/V) as mobile phase Berberin was quantified by ultraviolet absorbance at 349 nm. The method proved to be linear in the range of 1 2\|19 2 mg/L. The lower limit of detection of berberin was 0 6 mg/L. Intra\|day and inter\|day coefficients of variation of assay for berberin were 0 6%\|3 5%(n=5) and 5 3%\|6 5% (n=5), respectively. The recoveries of the method were 89 1%\|91 4%