摘要
通过BACTEC MGITTM 960全自动分枝杆菌培养鉴定/药敏仪对重组人干扰素α-2b-卡介苗(hIFN-α-2b-BCG)菌株进行利福平等10种抗菌药物的敏感性试验,以野生BCG作对照观察药物对重组BCG菌株活性的潜在影响.结果发现:重组人干扰素α-2b-卡介苗在系统感染上对喹酮类的氧氟沙星、环丙沙星敏感,对氨基糖苷类药物的庆大霉素和卡那霉素耐药.在抗结核类药物中,除对吡嗪酰胺耐药外,对利福平等其他几种抗生素均敏感.因尿液回收率高,几乎所有的抗菌药物都对膀胱中的菌株有影响.
To understand the situation of drug resistance of hlFN-α-2b BCG, in order to explore the curative effects of antibiotics on adverse reaction caused by IFN-α-2b-BCG during treating bladder carcinoma and their impact on an-ti-tumor effect of recombinant IFN-α-2b-BCG, wild BCG served as control, 10 kinds of antibacterial drugs including rifampicin, potentially effective against the IFN-α-2b-BCG, were measured in vitro by the BACTEC 960TB method, to observe the potential impact of antibiotics on the activity of recombinant BCG strains. The results showed that IFN-α-2b-BCG was susceptible to quinolones in systemic infections but resistant to some aminoglycosides. It was re- sistant to pyrazinamide but showed good susceptibility toward the other antituberculosis drugs tested. Most antibac- terials may interfere with IFN-α-2b-BCG in the bladder because of high urinary recovery.
出处
《天津师范大学学报（自然科学版）》
CAS
2012年第3期75-77,共3页
Journal of Tianjin Normal University：Natural Science Edition
基金
国家自然科学基金(30872587)
天津卫生局重点攻关项目(11KJ147)
天津市科技支撑计划重大项目(07ZCKFSH03200)
国家科技部"十一五"重大专项新药创建课题(2009ZX09103-699)
关键词
膀胱肿瘤
重组BCG
人干扰素Α-2B
免疫反应
耐药性
urinary bladder neoplasms
recombinant BCG vaccine
IFN-α-2b
immunotherapy
drug resistance
