期刊文献+

高雄山虫草人工栽培菌株筛选及搔菌对其子实体生物量的影响 被引量:5

Selection of an Anamorph of Cordyceps takaomontana for Artificial Cultivation,and Effect of 'Scratching' on Fruit Body Biomass
下载PDF
导出
摘要 以小麦粒固体培养基栽培高雄山虫草。筛选了适于生产的优良菌株,该菌株栽培后生长良好,搔菌后出草较多、整齐,但生物量和生物转化率与未搔菌相比没有明显差异;搔菌组第二潮子实体生物量和生物转化率均优于未搔菌组,搔菌组平均每瓶分别为(0.33±0.01)g和(2.2±0.01)%,未搔菌组分别为(0.12±0.01)g和(0.7±0.01)%。经过二潮子实体培养总生物量和总生物转化率高于一次培养。 An anamorphic strain of Cordyceps takaomontana,GZUIFR-PT123,was selected for artificial cultivation based on the production of higher numbers of uniform(less branching) fruit bodies on potato dextrose agar.GZUIFR-PT123 mycelium was grown at 25 ℃ in culture bottles(13 cm high,6 cm diameter) containing a substrate mix consisting of 15 g wheat seeds,4 g silkworm chrysalis meal,0.5 g maltose,10 mL mineral salts solution(0.1% CaCl2,0.5% ZnSO4 and 0.5% MgSO4),5 mg vitamin B1and 25 mL water.After the growth medium was fully colonized(8~10 d),the upper surface(0.3~0.5 cm) was removed("scratching") and discarded.At the first flush,no significant differences in fruit body production were recorded between bottles subjected to "scratching" treatment and controls in which the upper surface was retained.However,after scratching,GZUIFR-PT123 fruit bodies were more uniform,and biological efficiency values at the second flush was significantly higher in treated bottles(2.2%) compared with untreated controls(0.7%).
出处 《食用菌学报》 北大核心 2012年第2期39-42,共4页 Acta Edulis Fungi
基金 国家自然科学基金(编号:30960004 31093440) 贵州省教育厅自然科学基金(编号:2008059) 贵州大学引进人才科研项目(编号:2007-035)和贵州大学自然科学专项科研基金项目(编号:2010-102)的部分研究内容
关键词 高雄山虫草 搔菌 子实体生物量 生物转化率 Cordyceps takaomontana scratching treatment fruit body biomass biological efficiency
  • 相关文献

参考文献18

  • 1NAM KS, JO YS, KIM YH, et al. Cytotoxic activities of acetoxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes[J]. Life Sci, 2001, 69(2) :229-237.
  • 2LUANGSA-ARD J J, HYWEL-JONES NL, MANOCH L, et al. On the relationships of Paecilomyces sect. Isarioidea species [J]. Mycol Res, 2005, 109(5): 581-589.
  • 3LEE SH, HWANG HS, YUN JW. Production of polysaccharides by submerged mycelial culture of entomopathogenic fungus Cordyceps takaomontana and their apoptotic effects on human neuroblastoma cells[J]. Korean J Chem Eng, 2009, 26 (4): 1075-1083.
  • 4陈安徽,陈宏伟,邵颖,樊美珍.细脚拟青霉活性成分分析及清除自由基活性研究[J].食品科学,2009,30(13):25-28. 被引量:8
  • 5TSUSHIMA M, YAMAMOTO K, GORYO M, et al. Hot-water extract of Paecilomyces tenuipes from silkworm pupae improves D-galactose-induced brain aging in mice[J].J Insect Biotechnol Sericology, 2010, 79(2): 45-51.
  • 6SAPKOTA K, MOON SM, CHOI BS, et al. Enhancement of IL-18 expression by Paecilornycestenuipes[J]. Mycoscience, 2011, 52(4) :260-267.
  • 7韩燕峰,梁建东,邹晓,董旋,梁宗琦.高雄山虫草无性型研究进展[J].食用菌学报,2011,18(4):89-94. 被引量:16
  • 8LEE SM, PARK NS, JIN BR, et al. Effects of Paecilomyces tenuipes cultivated in egg yolk on lipid metabolism in rats on a high fat-cholesterol diet[J].J Med Food, 2006, 9(2):214-222.
  • 9XU CP, KIM SW, HWANG HJ, et al. Production of exopolysaecharides by submerged culture of an enthomopathogenic fungus, Paecilomyces tenuipes C240 in stirred-tank and airlift reactors [J]. Bioresour Technol, 2006, 97(5) : 770-777.
  • 10SAMSON RA. Paecilomyces and some allied hyphomycetes[J]. Stud Mycol, 1974, (6) :1-119.

二级参考文献41

共引文献68

同被引文献193

引证文献5

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部