北柴胡药材的HPLC指纹图谱研究

HPLC Fingerprint Chromatogram of Bupleurum chinense DC.Medicinal Materials

[目的]建立北柴胡药材的HPLC指纹图谱研究方法。[方法]采用Zorbax SB-C18(250 mm×4.6 mm ID,5μm)色谱柱,在柱温25℃、检测波长220 nm、流速为1.0 ml/min的条件下,以乙腈-0.05%磷酸水溶液为流动相对9个不同产地的柴胡样品进行梯度洗脱,分析其指纹图谱。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对9个不同批次的北柴胡药材指纹图谱进行相似度分析。[结果]各批北柴胡药材中均有11个特征峰,各峰分离度良好,各批次药材间共有峰的相对保留时间RSD均<1.0%,药材间相似度均>90%。[结论]该研究采用HPLC法,以柴胡皂苷a为内参比峰,建立了北柴胡药材的HPLC指纹图谱,方法稳定、重现性好,可有效控制北柴胡药材的质量。

[Objective] To establish HPLC fingerprint for Bupleurum chinense DC..[Method] The Bupleurum chinense DC.samples from nine regions were gradient eluted with an Agilent Zorbax SB-C18 column（4.6 mm×250 mm,5.0 μm）at 25 ℃ and a mobile phase consisting of 0.05% phosphoric acetonitrile（A） and water at a flow rate of 1.0 ml/min and at the detection wavelength of 220 nm,their fingerprints were analyzed.The similarity of the fingerprints was analyzed according to the software in Similarity Evaluation of the Fingerprints of Traditional Chinese Medicine（edition A of 2004） published by Chinese Pharmacopoeia Commission.[Result] 11 peaks were identified as common peaks.Each peak showed good separating degree,the RSDs of the relative retention rate of common peaks were below 1.0% with the similarity of the fingerprints of nine Bupleurum chinense DC.samples above 90%.[Conclusion] With saikoside a as internal reference peak,a HPLC fingerprint method was established for Bupleurum chinense DC.,the method is table and has good reproducibility,thus it can be used for the quality control of Bupleurum chinense DC..