罗布麻组织培养快繁技术体系研究

Research on Tissue Culture and Rapid Multiplication Techniques of Apocynum L.

[目的]研究罗布麻组织培养快繁技术,为其产业化栽培提供种苗来源。[方法]通过处理罗布麻种子获得无菌苗,切取无菌苗子叶、胚轴和茎尖置于不同激素配比的培养基上,比较不同激素浓度组合对子叶和胚轴分化、茎尖快繁、再生芽快繁和快繁苗生根的影响。[结果]子叶和胚轴分化形成再生芽的最佳培养基分别为MS+2.0 mg/L BA+0.03 mg/L NAA和MS+0.07 mg/L NAA;MS+2.0 mg/LBA+0.02 mg/L NAA为茎尖快繁的最佳培养基;MS+1.9 mg/L BA+1.7 mg/L NAA为再生芽快繁的最佳培养基;1/2MS+0.6 mg/LNAA为快繁苗的最佳生根培养基。[结论]该研究筛选出了罗布麻组培快繁技术体系的培养基激素配比,为罗布麻产业化栽培提供了技术保障。

[Objective] To study the tissue culture and rapid multiplication of Apocynum L.,so as to provide plantlet sources for its industrialized cultivation.[Method] The asepsis seedlings of Apocynum were obtained,and its cotyledons,hypocotyls and shoot tips were placed on the media which contained different hormone concentration.Finally,the influence of different hormone combinations on differentiation of cotyledons and hypocotyls,rapid multiplication of shoot tips,rapid multiplication of regenerated shoots,and rooting of test-tube plantlets was compared.[Result] MS＋2.0 mg/L BA＋0.03 mg/L NAA and MS＋0.07 mg/L NAA were the optimum media for inducing regenerated buds from cotyledons and hypocotyls respectively;MS＋2.0 mg/L BA＋0.02 mg/L NAA was the best medium for rapid multiplication of shoot tips;MS＋1.9 mg/L BA＋1.7mg/L NAA was the best medium for rapid multiplication of regenerated buds;and 1/2MS＋0.6 mg/L NAA was the best medium for inducing roots.[Conclusion] The optimum hormone combination was determined for Apocynum＇s rapid multiplication by tissue culture,which provides technical support for Apocynum industrialized cultivation.