MMP-9和TIMP-1在胃癌组织中的表达失衡及其与ZHX2的相关性

Correlation between MMP-9/TIMP-1 imbalance and ZHX2 expression in gastric carcinoma

目的:探讨胃癌中MMP-9和TIMP-1蛋白表达与ZHX2蛋白表达的关系,并分析其与胃癌患者临床病理特征的相关性及其临床意义.方法:应用免疫组织化学Envision二步法检测62例胃癌组织及癌旁正常胃组织中MMP-9、TIMP-1及ZHX2蛋白的表达.用SPSS13.0统计软件分析MMP-9和TIMP-1蛋白表达与ZHX2蛋白表达的相关性,并分析其与患者的临床病理特征的关系.结果:MMP-9蛋白在62例胃癌组织及癌旁正常胃组织中阳性表达率分别为66.13%(41/62)和29.03%(18/62)(P<0.01),TIMP-1蛋白在胃癌组织及癌旁正常胃组织中阳性表达率分别为41.94%(26/62)和74.19%(46/62)(P<0.01).MMP-9蛋白表达在ZHX2阳性和阴性表达组中的阳性率分别为:84.44%(38/45)和17.65%(3/17)(P<0.05),与患者的年龄、性别、肿瘤大小、发生部位、大体类型、分化程度、淋巴结转移、远处转移、TNM分期均无关(P>0.05),而与肿瘤的浸润深度有关(P<0.05);TIMP-1蛋白表达在ZHX2阳性和阴性组中阳性率分别为:28.89%(13/45)和76.47%(13/17)(P<0.05),与患者的年龄、性别、肿瘤大小、发生部位、大体类型、分化程度、浸润深度、淋巴结转移、远处转移、TNM分期均无关(P>0.05).结论:MMP-9高表达可以作为评估胃癌恶性生物学行为及预后的指标,MMP-9/TIMP-1表达失衡与ZHX2表达相关,MMP-9和TIMP-1可为临床选择化疗药物提供参考依据.

AIM： To detect the expression of matrix metal- loproteinase 9 （MMP-9）, tissue inhibitor of me- talloproteinase-1 （TIMP-1） and ZHX2 in gastric carcinoma and to analyze their association with pathological features. METHODS： Immunohistochemistry was used to examine the expression of MMP-9, TIMP-1, and ZHX2 proteins in 62 cases of gastric carcinoma and matched tumor-adjacent tissue specimens. The correlation of MMP-9, TIMP-1 and ZHX2 pro- tein expression with clinicopathological character- istics of gastric carcinoma was then analyzed. RESULTS： The positive rate of MMP-9 expres-sion was significantly higher （66.13% vs 29.03%, P 〈 0.01）, and that of TIMP-1 was significantly lower （41.94% vs 74.19%, P 〈 0.01） in gastric carcinoma than in tumor-adjacent tissue. The positive rate of MMP-9 expression was signifi- cantly higher in the ZHX2-positive group than in the ZHX2-negative group （84.44% vs 17.65%, P 〈 0.05）. MMP-9 expression was not associated with age, gender, tumor size, tumor location, general type, tumor differentiation, lymph node metastasis, distant metastasis, or TNM stage, but was significantly associated with depth of invasion （P 〈 0.05）. The positive rate of TIMP-1 expression was significantly lower in the ZHX2- positive group than in the ZHX2-negative group （28.89% vs 76.49%, P 〈 0.05）. TIMP-1 expression was not associated with age, gender, tumor size, tumor location, general type, tumor differentia- tion, depth of invasion, lymph node metastasis, distant metastasis, or TNM stage.CONCLUSION： Detection of MMP-9 protein ex- pression may be used to assess the malignant biological behavior and prognosis of gastric carcinoma. MMP-9/TIMP-1 imbalance may be related to the expression of ZHX2 in gastric car- cinoma,