五指山小型猪胰岛对糖尿病大鼠的治疗作用

Treatment of diabetic rats using islets from a herd of outbred Wuzhishan miniature pigs

目的:探讨小型猪胰岛治疗糖尿病大鼠的作用和功效.方法:成年健康♂五指山小型猪和普通家猪作为胰岛供体.胰腺获取后经胶原酶消化后进行胰岛提取纯化,纯化后的胰岛通过门静脉移植到糖尿病SPF大鼠的肝内,术后每天肌注环孢菌素(20mg/kg)作为免疫抑制剂,并通过检测糖尿病大鼠的血糖变化和肝脏组织学检查来判定胰岛移植后的存活情况和纠正胰岛移植的效果.结果:五指山小型猪胰腺消化后胰岛产量为4608 IEQ/g±593 IEQ/g,纯化后为3820 IEQ/g±718 IEQ/g,纯度为85%,取自屠宰场猪胰岛产量为纯化前为3500 IEQ/g±625 IEQ/g,纯化后为2720 IEQ/g±435 IEQ/g,纯度为80%.两组84.6%糖尿病大鼠在移植术后第1天,血糖降至正常.维持时间:小型猪胰岛术后存活时间为3-5d(中位存活时间:4.5d),普通家猪胰岛存活时间为2-4d(中位存活时间:3.7d).经Kaplan-Meier分析,两组胰岛存活时间无差别.结论:封闭群五指山小型猪分离纯化后的胰岛产量高,功能良好,可以纠正糖尿病大鼠的高血糖,适合作为异种胰岛移植的理想供体.

AIM： To assess the efficacy of Wuzhishan minia- ture pig islets in the treatment of diabetic rats. METHODS： Adult male miniatures pigs and market pigs were used as donors. After col- lagenase digestion, the islets were purified and intraportally transplanted into diabetic SPF rats, with cyclosporine （20 mg/kg） intramuscularly injected as an immunosuppressive agent. Isletgraft survival was monitored by the detection of changes in blood sugar and liver histology in diabetic rats. RESULTS： Islet yield was 4 608 IEQ/g ＋ 593 IEQ/g in the miniature pig group and 3 820 IEQ/g ＋ 718 IEQ/g in the market pig group. On day 1 after transplantation, the blood glucose de- creased to normal in 84.6% of diabetic rats in both groups. Islet survival time was 3-5 d （me- dian： 4.5 d） in the miniature pig group and 2-4 d （median： 3.7 d） in the market pig group. Kaplan- Meier analysis showed no significant difference between the two groups. CONCLUSION： The Wuzhishan miniature pigs are an ideal donor species for islet xenotransplanta- tion due to high islet yield and good islet function.