Twist1和基质金属蛋白酶2在子宫内膜样腺癌组织中的表达及意义

Expression and significance of Twist1 and MMP-2 in endometrial endometrioid adenocarcinoma

【摘要】目的探讨Twistl和基质金属蛋白酶2（MMP－2）蛋白在子宫内膜样腺癌组织中的表达及其相互关系。方法建立组织微阵列平台，应用免疫组织化学方法检测70例子宫内膜样腺癌组织中Twistl和MMP-2蛋白的表达情况。结果Twistl蛋白主要定位于肿瘤细胞核，而MMP-2蛋白主要定位于肿瘤细胞质。70例子宫内膜样腺癌组织中，Twistl的高表达率为65．7％，MMP-2的阳性率为67．1％。Twistl表达与临床分期、肿瘤子宫肌层浸润和卵巢转移有关（均P〈0．05），MMP．2表达与临床分期、肿瘤分级和肿瘤子宫肌层浸润有关（均P〈0．05）。相关性分析显示，Twistl表达与MMP-2表达呈显著正相关（r=0．328，P〈0．01）。Twistl高表达患者的总生存率（80．4％）和无复发生存率（78．3％）均低于Twistl低表达患者（均100．0％，均P〈0．05）。多因素分析显示，临床分期和淋巴结转移是影响子宫内膜样腺癌患者预后的独立危险因素（均P〈0．05），而Twistl和MMP-2表达与子宫内膜样腺癌患者的预后无关（均P〉0．05）。结论子宫内膜样腺癌中Twistl和MMP-2的表达与肿瘤侵袭和转移有密切关系；MMP-2表达可能在一定水平上受到Twistl的调控。

Objective To investigate tile expression of Twistl and MMP-2 protein and their significance in endometrial endometrioid adenocarcinoma. Methods The expression of Twistl and MMP-2 protein in 70 cases of endometrial endometrioid adenocarcinoma was detected on tissue chips using immunohistochemical staining. Results The positive rates of Twistl and MMP-2 protein expression were 65.7% and 67.1%, respectively. Both of the high expressions of Twistl and MMP-2 were positively correlated with FIGO staging and tumor myometrial invasion （P 〈 0. 05, respectively）. Also the high expression of Twistl was positively correlated with ovarian metastasis and the expression of MMP-2 was positively correlated with tumor grading （P 〈 0.05 ）. The patients＇overall survival and relapse-free survival in the group of high Twistl expression were shorter than that in the group of low Twistl expression （P 〈 0.05）. The expression of MMP-2 was positively correlated with Twistl expression （P 〈 0.01, respectively）. Conclusions The expression of Twistl may be closely correlated with the tumor invasion, metastasis and prognosis in patients with endometrial endometrioid adenocarcinoma. The expression of Twistl has a close relationship with MMP-2 in endometrial endometrioid ad