摘要
目的探讨非小细胞肺癌(NSCLC)组织中促红细胞生成素(EPO)及其受体(EPO—R)的表达,及其与新生血管形成的关系。方法采用免疫组织化学法检测31例NSCLC组织和癌旁组织中EPO和EPO—R的表达情况,以21例肺良性病变组织作为对照,分析EPO和EPO—R表达与各临床病理因素的关系。以CD34标记血管内皮细胞,计算微血管密度(MVD)。结果EPO和EPO-R在NSCLC组织中的阳性率分别为67.7%和96.8%,在癌旁组织中阳性率分别为19.4%和35.5%,在肺良性病变组织中的阳性率分别为9.5%和19.O%(P=0.000)。EPO和EPO—R表达与NSCLC患者的临床分期、病理类型、组织分化程度及淋巴结转移无关(P〉0.05)。31例NSCLC患者中,EPO阳性者的MVD为26.14±5.26,显著高于EPO阴性者(15.97±8.29;t=5.34,P〈0.001);EPO—R阳性者的MVD为27.13±6.33,显著高于EPO—R阴性者(11.72±5.130;t=7.46,P〈0.1301)。MVD与NSCLC分化程度有关,高、中分化的NSCLC组织中MVD低于低分化者(P=0.025),MVD与NSCLC分期有关,临床分期越晚,MVD越高(P〈0.001)。结论EPO和EPO—R在NSCLC组织中高表达,可能参与了肿瘤的发生过程;EPO/EPO—R的表达与NSCLC血管生成程度及病情进展有关,可为临床诊断提供依据。
Objective Erythropoietin and erythropoietin receptor (EPO-R) are expressed in many kinds of tumors. The EPO/EPO-R signaling is involved in tumor cell proliferation, invasion and angiogenesis. The aim of this study was to detect the expression of EPO-R in non-small cell lung cancer (NSCLC) , and explore its correlation with angiogenesis. Methods The expression patterns of EPO and EPO-R in 31 cases of NSCLC tissues were detected by immunohistochemistry, and that in benign lung lesions of 21 patients as control. To analyze the correlation of EPO/EPO-R expression patterns and clinicopathological factors. CD34 was used to label the vascular endothelial cells and calculate the microvessel density (MVD). Results The positive rates of EPO and EPO-R expression in NSCLC were 67.7% and 96.8% , respectively, significantly higher than those in the control ones. The positive rates of EPO and EPO-R expression in adjacent tissues were 19.4% and 35.5% , and in benign lesions were 9.5% and 19.0%, respectively (P 〈0. 001 ). The expression patterns of EPO/EPO-R were not related with pTNM stage, histological type, histological grade and lymph node metastasis (P 〉 0.05 ). Increased MVD was correlated with poor differentiation, lymph node metastasis, and advanced stage. Conclusions High expression of EPO/EPO-R in NSCLC patients suggest that they may be involved in tumorigenesis. EPO/ EPO-R expression and MVD are closely related, and they might be an endogenous stimulant of angiogenesis during the progression of non-small cell lung cancer. It may provide evidence for clinical diagnosis.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2012年第8期605-608,共4页
Chinese Journal of Oncology
基金
新疆维吾尔自治区自然科学基金(2010211A30)