RP-HPLC法测定射干中射干苷及次野鸢尾黄素的含量

Determination of Contents of Tecteridin、Irisflorerntin in Rhizoma Belamcandae by RP-HPLC

目的建立同时测定射干中射干苷及次野鸢尾黄素含量的高效液相分析方法。方法采用C18柱(4.6×150mm,5μm),以甲醇-0.007mol.L-1磷酸二氢钾水溶液为流动相,检测波长为266nm;流速:1.0ml.min-1;柱温(25±1)℃。结果射干样品中射干苷及次野鸢尾黄素平均加样回收率分别为98.77%(RSD=2.73%),95.98%(RSD=2.39%)。结论本方法可靠、简便、快速,可用于射干药材含量测定,为合理开发利用射干药用资源及其质量评价提供实验依据。

OBJECTIVE To establish an RP-HPLC method to determine the contents of Tecterdin,Irisflorerntin in Rhizoma Belamcandae simultaneously.METHODS The RP-HPLC condition was Cls column, methanol - water （24：76 ,containing 0.007mol·L-1 KH3PO4） as mobile phase and 266 nm as detective wavelength, the flow rate of 1.0 mL·min-1 and the temperature at （25±1）℃. RESULTS The average recoveries were 98.77%（RSD=2.73%）,95.98%（RSD=2.39%） for Tecteridin,Irisflorerntin in Rhizoma Belamcandae Sample,respectively. CONCLUSION The method is accurate ,simple and rapid. It can be used for the quality control of Rhizoma Belamcandae and providing a experimental basis for development its resources.