胶原/细菌纤维素复合材料的细胞相容性评价

Evaluation of the cytocompatibility of collagen/bacterial cellulose nanocomposites

目的评价胶原/细菌纤维素复合材料的细胞相容性。方法将胶原(collagen,Col)和细菌纤维素(bacterial cellulose,BC)通过物理交联法和化学交联法制备成Col/BC物理组和Col/BC化学组,以BC为对照组,采用SEM(扫描电镜)观察材料形貌。采用小鼠胚胎成骨细胞株(MC3T3-E1)与材料复合培养,分为空白对照组(等量的细胞悬液)、材料对照组:BC、实验组1:Col/BC物理组、实验组2:Col/BC化学组观察细胞生长状况,MTT(四氮唑盐)观测细胞在1、2、3、4、5、6 d后的增殖情况,细胞与材料复合第48 h,SEM观察细胞粘附形貌。结果 SEM观察在BC纤维上均匀包覆了一层蛋白质,MTT检测结果表明3 d后Col/BC(物理交联组)组高于其他组,实验组与材料对照组:BC相比,差异有统计学意义(P<0.05)。SEM观察在细胞与材料复合培养48 h,纤维表面均有少量成骨细胞粘附。结论与材料对照组:BC相比,Col/BC物理组和Col/BC化学组均具有良好的细胞相容性,其中,Col/BC物理组表现出更好的细胞相容性。

Objective To evaluate the cell compatibility of collagen/bacterial cellulose composites Methods The Col/BC composites were prepared by physical and chemical cross linking of Col and BC, where BC was assigned as the control group and SEM was used for morphological observation. The embry- onic osteoblast cells of mice are co-cultured with the BC, Col/BC physical group, Col/BC chemical group and control group, respectively, and examined by MTT and SEM. Results SEM showed that the bacterial cellilose was uniformly coated with protein. MTT showed that the Col/BC physical group was better than the other groups after 3 days. SEM observed adhesion of a few ceils. Conclusions Both Col/BC composites prepared by physical and chemical cross linking show good cytocompatibihy, and the physically prepared is even better.