无镁诱导培养大鼠海马神经元与SH-SY5Y细胞自发性放电的变化

The Spontaneous Discharge of Cultured Rat Hippocampal Neurons and SH-SY5Y Cells Induced by Mg^(2+)-free Extracellular Fluid

目的利用无镁细胞外液诱导原代培养大鼠海马神经元和SH-SY5Y细胞建立癫痫放电模型。方法采用新生24 h内Wistar大鼠,分离海马神经元进行原代培养。无镁细胞外液处理体外培养至10 d的神经元和传代培养的SH-SY5Y细胞3 h后恢复正常细胞外液,应用全细胞膜片钳技术记录2种细胞的放电情况。结果无镁处理后的神经元存在自发性的"癫痫样"放电;SH-SY5Y细胞未出现"癫痫样"放电。结论细胞间通过突触联系形成网状结构可能是诱导"癫痫样"放电的必要条件之一。

Objective To detect the spontaneous discharge of cultured rat hippocampal neurons and SH-SYSY cells in Mg2＋-free extracellu- lar fluid. Methods The postnatal hippocampal tissues were taken from 1-day-old Wistar rats and were used for primary culture. Neurons after 10-day culture and SI-I-SY5Y cells were treated with Mg2＋-free exta＇acellular fluid for 3 h, then the discharge activity was recorded by patch clamp technique. Results Neurons showed spontaneous ＂epileptiform discharge＂ after the Mg2＋-free extraceUular fluid treatment; on the contrary,SH-SY5Y cells did not show spontaneous ＂epileptiform discharge＂. Conclusion Synaptic connections between cells through the formation of network stnacture mac be necessary for inducing ＂epileptiform discharge＂.