标本放置时间对凝血酶原时间和活化的部分凝血活酶时间检测结果的影响

Effect of Storage Time on Prothrombin Time and Activated Partial Thromboplastin Time Test on Abnormal Specimens

目的探讨凝血功能异常时,标本放置时间对凝血酶原时间（PT）和活化的部分凝血活酶时间（APTT）检测结果的影响。方法利用STA-R全自动血凝仪及配套试剂,测定不同放置时间下70例血凝标本PT和APTT的检测结果。根据PT和APTT的正常参考值,将空腹（0 h）检测结果分为正常组（PT：11.0~14.3 s,APTT：32.0~43.0 s）和异常组（PT〉14.3 s,APTT〉43.0 s）。将标本于室温下分别放置1、2、4、6、8、12、24 h,检测其PT和APTT,对结果进行统计学分析。结果正常组PT与APTT标本分别于室温存放8 h和4 h时检测结果基本稳定。异常组PT与APTT标本分别于室温存放4 h和2 h时检测结果基本稳定。异常组较正常组检测结果更易受标本放置时间的影响,PT标本放置6 h、APTT标本放置4 h时,检测结果即发生明显的改变。结论对于凝血异常者,标本放置时间对检测结果影响甚大,PT和APTT测定均应于标本采集后2 h内尽快进行。

Objective To investigate the effect of storage time on pmthrombin time （PT） and activated partial thmmboplastin time （AP3T） at room temperature under the condition of coagulation disorder. Methods Clinical specimens with normal and prolonged storage time on PT and APTT tests were enrolled. The assay was conducted immediately after centrifuge,and PT of 11.0-14.3 s and APTF of 32.0-43.0 s were considered as the normal range. The specimens were kept at room temperature, and the coagulation test was performed at 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h after collection. Results For normal specimens, PT result was stable up to 8 h, APIT up to 4 h at room temperature; but for the prolonged storage time, PT was stable up to 4 h, APTT 2 h. Conclusion Normal specimens were stable at 4 h for coagulation tests, but the coagulation assays should be performed in 2 h for the prolonged group was stable for only 2 h.