地拉罗司对大鼠窄蒂缺氧皮瓣模型促进微血管生成的实验

Mimic hypoxia of deferasirox in improving growth of micrangium in a narrow pedicle flap

目的探索术前应用地拉罗司（deferasirox）对大鼠乒乓拍样狭长窄蒂缺氧皮瓣模型微血管生成的促进作用及与用约时间的相关性。方法成年雄性SD大鼠40只．随机分为对照组（8只），实验组（32只，分为1、3、5、7d组，每组8只）。实验组：地拉罗司100mg／kg分别连续灌胃1、3、5、7d；对照组：生理盐水100mg／kg连续灌胃7d，灌胃结束后，次日行乒乓拍样窄蒂任意型皮瓣成形术，均于术后第7天取材，应用免疫组织化学、Western印迹及实时荧光定量聚合酶链式反应（PCR）法检测CD34表达，应用实时荧光定量PCR检测低氧诱导因子-1α（HIF1α）和血管内皮生长因子（VEGF）的表达。结果实验组较对照组皮瓣成活率明显提高，随着用药时间的增加，皮瓣微血管密度（MVD）、HIF1α和VEGF均逐渐增高，7d达最高。结论地拉罗司能诱导HIF-1α的分泌，促进VEGF产生．增加MVD表达，明显具有提高皮瓣对低氧的耐受，减少内皮细胞低氧后的坏死率，对缺血缺氧的窄蒂皮瓣具有较好的保护作用。

Objective To study the function of hypoxia mimics of different exposure time of de- ferasirox in improving the growth of micrangium in a narrow pedicle flap. Methods Fourty male SD rats were divided into 2 groups： experimental group was fed with deferasirox 100 mg/kg per day from ld. 3d, 5d and 7d, respectively, before the surgery of transferring the narrow pedicle flap, while con-trol group iust fed with saline. After 7 days, the immunohistochemistry, Western blot and quantita-tive reverse transcription PCR （qPCR） were performed to examine the expression of CD34. qPCR was used to examine the expression of HIF lα, VEGF in order to investigate the regulatory effect of de- ferasirox in improving the growth of micrangium and the distinction among the differeat exposed time of deferasirox in the narrow pedicle flap. Results The deferasirox group exhibited a marked improve-ment in flap healing time. and with the increasing administration time of deferasirox, the expression of MVD, HIF 1α and VEGF was improved in each treated group （P〈0.05）. Conclusions Deferasirox can induce HIF l a secretion and increase CD34 expression, and so deferasirox can protect endothelial cells from hypoxic and ischaemic injury.