甲型H1N1流感患者Th1/Th2细胞因子水平的观察

The Observation of Th1/Th2 Cytokine Levels in Influenza A(H1N1)

目的通过检测甲型H1N1流感患者血清中细胞因子的水平,初步了解重症甲型H1N1流感患者的免疫应答特点。方法收集2009年11月14日到2009年12月31日于中国医科大学附属盛京医院就诊并确诊为甲型H1N1流感患者作为病例组,同时随机选择同时期的健康体检者作为对照组,采用ELISA法分别检测2组血清干扰素γ(IFN-γ),白细胞介素4(IL-4)及白细胞介素10(IL-10)浓度。结果与对照组相比,IFN-γ及IL-10均显著增高(P<0.05)。IL-4显著降低(P<0.05)。结论在甲型H1N1流感病毒感染的进程中,Th1和Th2细胞的平衡被打破,患者细胞免疫相对增强,体液免疫不同程度的受到抑制。

Objective To study the characteristics of immune response in influenza A （H1N1） patients by detecting the level of different cytokines. Methods Patients diagnosed as H1N1 virus infection from November 14 to December 31,2009 admitted in Shengjing Hospital of China Medical University were collected. Healthy individuals were random selected in the same time period as control group. The concen- tratians of interferon-γ （IFN-γ）, interleukin-4 （ IL-4 ） and interleukin- 10 （ IL- 10 ） were examined by serum enzyme-linked immunosorbnent assay （ELISA）. Results Compared with the control group,the IFN-γand IL-10 level in serum of H1N1 patients were significantly higher （P 〈 0.05 ）, whereas IL-4 level was significantly lower （P 〈 0.05）. Conclusion The influenza A（H1N1 ） infected patients showed an im- balance of Thl and Th2 cytokines ,there was a relatively enhanced cell mediated immunity and attenuated humoral immune response.