“双固一通”电针治疗对老年阳虚大鼠淋巴细胞凋亡相关基因表达的影响

Effect of Electroacupuncture on Spleen Lymphocyte Apoptosis-related Gene Expression in Aging Rats with Yang Deficiency

目的:探讨"双固一通"电针治疗对老年阳虚模型大鼠淋巴细胞凋亡相关基因Fas mRNA、BaxmRNA、Bcl-2mRNA表达的影响。方法:选用5月龄SD雌性大鼠30只,随机分为正常对照组、老年阳虚模型组、"双固一通"电针组,除正常对照组外,其余2组大鼠皮下注射D-半乳糖40d后再肌肉注射氢化可的松7d制作老年阳虚大鼠模型。"双固一通"电针组取"关元""后三里""百会",每周治疗6次,共4周。采用实时荧光定量RT-PCR技术检测各组大鼠脾脏促凋亡基因Fas mRNA、Bax mRNA以及抗凋亡基因Bcl-2mRNA的表达。结果:老年阳虚模型大鼠促凋亡基因Fas mRNA、Bax mRNA表达水平明显高于正常对照组,抗凋亡基因Bcl-2mRNA表达下调(均P<0.01);与老年阳虚模型组相比,"双固一通"电针组大鼠促凋亡基因Fas mRNA、Bax mRNA表达明显下调,抗凋亡基因Bcl-2mRNA表达上调(P<0.05,P<0.01)。结论:"双固一通"电针法能有效下调老年阳虚模型大鼠促凋亡基因Fas mRNA、Bax mRNA表达,上调抗凋亡基因Bcl-2mRNA的表达,这可能是"双固一通"电针法对老年阳虚大鼠淋巴细胞凋亡相关基因表达的调控模式。

Objective To observe effects of electroacupuncture （EA） of ＂Guanyuan＂ （GV 4）-＂Housanli＂ （ST 36）- ＂Baihui＂ （GV 20,＂Shuanggu Yitong＂ acupoint prescription） on the expression of splenic lymphocyte apoptosis-related genes Fas, Bax, and Bcl-2 in aging rats with yang deficiency. Methods Thirty Sprague-Dawley aged female rats were randomly and equally divided into normal control, yong deficiency model （model） and EA groups. The yang deficiency model was established by subcutaneous injection of D-galactose （125 mg/kg） and hydrocortisone（ 1.5 mg/100 g）. EA （2 Hz, 1 mA） was applied to ＂Guanyuan＇（GV 4）, ＂Housanli＇（ST 36） and ＂Baihui＂（GV 20） for 15 min, once daily, 6 times per week for 4 weeks. The expres- sion of pro-apoptotic genes Fas, Bax and anti-apoptosis gene Bcl-2 in the spleen tissues were detected by real-time quantitative RT-POR. Results In comparison with the normal control group, splenic Fas mRNA and Bax mRNA expression levels were upregulated significantly, and Bcl-2 mRNA expression level was down-regulated considerably in the model group（ P〈0.01 ）. After EA intervention, splenic Fas mRNA and Bax mRNA expression levels were down-regulated markedly, while Bcl-2 mRNA expression up-regulated remarkably in the EA group（P〈0.05, P〈0. 01）. Conclusion EA of acupoint prescription [＂Guanyuan＂ （GV 4）, ＂Housanli＂（ST 36） and ＂Baihui＂（GV 20）] can down-regulate spleen Fas mRNA and Bax mRNA expression, and up-regulate Bcl-2 mRNA expression in aging rats with yeng deficiency, which may contribute to its effect in improving yeng deficiency in clinic.