“烧山火”与“透天凉”针法对热证模型家兔肛温及血清内毒素含量的影响

Effects of Heat and Cool-producing Needling Manipulations on Rectal Temperature and Serum Endotoxin Content in Endotoxin-induced Heat Syndrome Rabbits

目的:比较传统补泻手法"烧山火""透天凉"对热证模型家兔的影响。方法:日本大耳白兔24只,随机分为空白对照组、模型组、烧山火组、透天凉组。腹腔注射大肠杆菌内毒素(ET)制造热证模型。治疗组动物选取"曲池"穴,分别施以"烧山火"补法和"透天凉"泻法各1次。用体温计检测各组家兔基础肛温及治疗前后肛温,用鲎试剂检查法检测血清ET含量。结果:造模后家兔肛温和血清ET含量增高(均P<0.01)。治疗两组与模型组相比肛温显著降低(P<0.05,P<0.01),ET含量均显著降低(P<0.01),透天凉组ET含量较烧山火组下降更明显(P<0.01)。结论:"烧山火""透天凉"确能降低实热模型家兔血清ET含量,且"透天凉"退热效果更佳。

Objective To observe the effect of traditional manipulations of ＂Shaoshanhuo＂（heat-producing needling） and ＂Toutianliang＇（cool-producing needling） on body temperature and serum endotoxin level in heat syndrome rabbits. Methods Twenty-four Japanese rabbits were randomly divided into control, model, Shaoshanhuo and Toutianliang groups. Heat-syndrome model was established by subcutaneous injection of bacterium coil endotoxin solution （40 μg/mL, 2 mL/kg）. Heat-producing and cool-producing needling was applied to bilateral ＂Quchi＂ （LI 11） for 5 min, respectively. Rectal temperature was detected by using a thermometer, and serum endotoxin content assayed by using Limulus Ameboyte Lysate kit （luminescence measuring）. Results In comparison with the control group,both rectal temperature and serum endotoxin levels were increased significantly in the model group（P〈0.01）. While compared to the model group, the rectal temperature and serum endotoxin levels were down- regulated considerably in both Shaoshanhuo and Toutianliang groups（P〈0.05, P〈0.01）. The effect of the Toutianliang group was obviously superior to that of the Shaoshanhuo group in reducing serum endotoxin content （P〈0.01）. Conclusion Both heat-producing needling and cool-producing needling can lower rectal temperature and serum endotoxin levels in heat-syndrome rabbits, and the effect of cool-producing needling is relatively better in reducing endotoxin content.