摘要
目的:建立测定灵芪蠲肝胶囊中阿魏酸含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex C1(8250mm×4.6mm,5μm),流动相为乙腈-1%冰醋酸(32:68),流速为1.0mL·min-1,检测波长为320nm,柱温为30℃。结果:阿魏酸的检测浓度在9.84~98.40μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9994);平均加样回收率为99.12%,RSD=0.28%(n=9)。结论:本方法简便、易行,精密度、重复性、稳定性好,适用于灵芪蠲肝胶囊中阿魏酸的含量测定。
OBJECTIVE: To establish a method for the content determination of ferullic acid in Lingqi juangan capsules. METHODS: HPLC method was adopted. The determination was performed on Phenomenex C18(250 min×4.6 mm, 5 μm) column with mobile phase consisted of acetonitrile-1% glacial acetic acid (32:68) at the flow rate of 1.0 mL.min^-1. The detection wave- length was set at 320 nm and the column temperature was at 30 ℃. RESULTS : The linear range of ferullic acid were 9.84-98.40 μg.mL^-1(r=0.999 4) with mean recoveries of 99.12% (RSD=0.28% ,n=9). CONCLUSION: The established method is simple, easy, precise and reproducible with good stability, and it is suitable for the content determination of ferullic acid in Lingqi juangan capsules.
出处
《中国药房》
CAS
CSCD
2012年第31期2942-2943,共2页
China Pharmacy