HPLC法测定灵芪蠲肝胶囊中阿魏酸的含量

Content Determination of Ferullic Acid in Lingqi Juangan Capsules by HPLC

目的:建立测定灵芪蠲肝胶囊中阿魏酸含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex C1(8250mm×4.6mm,5μm),流动相为乙腈-1%冰醋酸(32:68),流速为1.0mL·min-1,检测波长为320nm,柱温为30℃。结果:阿魏酸的检测浓度在9.84～98.40μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9994);平均加样回收率为99.12%,RSD=0.28%(n=9)。结论:本方法简便、易行,精密度、重复性、稳定性好,适用于灵芪蠲肝胶囊中阿魏酸的含量测定。

OBJECTIVE： To establish a method for the content determination of ferullic acid in Lingqi juangan capsules. METHODS： HPLC method was adopted. The determination was performed on Phenomenex C18（250 min×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-1% glacial acetic acid （32：68） at the flow rate of 1.0 mL.min^-1. The detection wave- length was set at 320 nm and the column temperature was at 30 ℃. RESULTS ： The linear range of ferullic acid were 9.84-98.40 μg.mL^-1（r=0.999 4） with mean recoveries of 99.12% （RSD=0.28% ,n=9）. CONCLUSION： The established method is simple, easy, precise and reproducible with good stability, and it is suitable for the content determination of ferullic acid in Lingqi juangan capsules.