Q开关Nd:YAG激光与强脉冲光对鼠皮肤热休克蛋白70表达的影响

Effect of Q-switched Nd:YAG laser and intense pulsed light on the expression of heat shock protein 70 in rat skin

目的:确定Q开关1064 nm Nd:YAG激光与强脉冲光(IPL)对Wistar大鼠皮肤热休克蛋白70(HSP70)表达的影响。方法:Wistar大鼠20只,背部皮肤脱毛,平均分成3部分:近头侧区为正常对照组,中间区为IPL组,近尾侧区为YAG组。于照射后1天、1周、4周取大鼠皮肤标本,免疫组化技术检测HSP70的表达。结果:免疫组化显示:Q开关1064 nm Nd:YAG激光和IPL照射后1周,HSP70表达最为显著;照射后4周,HSP70表达下调,IPL组表达与正常对照组无差别,YAG组仍较正常对照组表达增强(P<0.05);两光照组间于照射后1天、1周和4周HSP70表达差别有统计学意义(P<0.05)。结论:HSP70的表达强度与光照后组织热损伤的程度和深度有关,Q开关1064 nm Nd:YAG激光对大鼠皮肤的非剥脱性嫩肤作用较IPL强且持久。

Objective： To determine the effect of Q - switched Nd：YAG laser and intense pulsed light （IPL） on the expression of heat shock protein 70 （HSPT0） in rat skin. Methods： The dorsal skin of twenty Wister rats were divided into three areas before treatments： the cephalic region as normal control group, the middle region as the IPL treatment group and the caudal region as the YAG group. The skin samples were taken at day 1, day 7 and on week 4 after irradiation. The expression of HSP70 protein was detected with immunohistochemistry. Results： The expression of HSP70 of two treatment groups were reached the most significant at 1 week. At week 4 after irradiation, the expression of HSP70 decreased and there was no difference between the IPL group and the control group （ P 〉 0.05）, but YAG group was still stronger than the control group （ P 〈 0.05）, The expression of HSP70 of two light irradiated groups were different significantly at 1 day, 1 week and 4 weeks （P 〈 0.05）. Conclusion： The intensity of HSP70 expression in rat skin is related to the degree and depth of thermal damage after the light irradiation. The effect of Q - switched 1064 nm Nd： YAG laser on rat skin rejuvenation is stronger and more durable than IPL.